Cytotechnology

, Volume 64, Issue 2, pp 139–144

Optimization of condition(s) towards establishment of primary islet cell cultures from WNIN/Ob mutant rat

  • V. Venkatesan
  • M. Chalsani
  • S. S. Nawaz
  • R. R. Bhonde
  • S. S. Challa
  • G. Nappanveettil
Brief Report

DOI: 10.1007/s10616-011-9409-y

Cite this article as:
Venkatesan, V., Chalsani, M., Nawaz, S.S. et al. Cytotechnology (2012) 64: 139. doi:10.1007/s10616-011-9409-y

Abstract

WNIN/Ob, a mutant rat strain, developed at the National Center for Laboratory Animal Sciences (NCLAS) facility of National Institute of Nutrition (NIN), is a new animal model to study the metabolic syndrome. These animals have 47% fat in their body and isolation of islets from these animals were compounded due to the formation of amorphous viscous and jelly like material which reduced the islet yield. However, islets isolated from WNIN adult (≥12 months) control rats gave a good islet recovery, under standard isolation procedures using collagenase digestion. In the present study we optimized culture conditions in WNIN/Ob rats to isolate islets with higher yield, and also established primary islet cell cultures from these mutant rats, retaining cellular integrity and functionality.

Keywords

Obese rats Collagenase Islet isolation Islet cell culture Viability and islet function 

Abbreviations

WNIN

Inbred Wistar rat strain established at NIN

WNIN/Ob

Wistar rats raised in the National Institute of Nutrition, with obesity trait

NCLAS

National Center for Laboratory Animal Sciences

NIN

National Institute of Nutrition

DTZ

Dithiazone

KRBH

Krebs–Ringer bicarbonate HEPES

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • V. Venkatesan
    • 1
  • M. Chalsani
    • 1
  • S. S. Nawaz
    • 1
  • R. R. Bhonde
    • 2
  • S. S. Challa
    • 1
  • G. Nappanveettil
    • 1
  1. 1.National Centre for Laboratory Animal Science, National Institute of NutritionJamai OsmaniaHyderabadIndia
  2. 2.Manipal Institute of Regenerative MedicineBangaloreIndia

Personalised recommendations