Abstract
Mercury, a xenobiotic metal, is a highly deleterious environmental pollutant. Moreover, in any form mercury is reported to be toxic. On the other hand, Thymbra spicata L., a member of the Lamiaceae family, has long been investigated popularly of biological roles; mainly antimicrobial and antioxidant activities. However, there are very scarce data on the cytogenetic effects of thyme species. The purpose of this study was to investigate the genetic safety of different extracts from T. spicata (water extract, methanol extract, and ethanol extract) and the effects of T. spicata on mercury (as HgCl2) induced genotoxicity. Sister chromatid exchange (SCE) and micronucleus (MN) assays were performed to assess DNA damages in cultured human lymphocytes (n = 5). Our results clearly revealed that, the SCE and MN rates induced by HgCl2 were alleviated by the presence of T. spicata. As conclusion, this study demonstrated for the first time that the T. spicata provided increased resistance of DNA against HgCl2 induced genetic damage in human lymphocytes. Based on the results of this study, it may be concluded that the T. spicata is a nontoxic material that could be used as a suppressor of heavy metal-induced genotoxicity.
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Abdalla FH, Belle LP, De Bona KS, Bitencourt PE, Pigatto AS, Moretto MB (2010) Allium sativum L. extract prevents methyl mercury-induced cytotoxicity in peripheral blood leukocytes (LS). Food Chem Toxicol 48:417–421
Aboul-Soud MAM, Al-Othman AM, El-Desoky GE, Al-Othman ZA, Yusuf K, Ahmad J, Al-Khedhairy AA (2011) Hepatoprotective effects of vitamin E/Selenium against Malathion—ınduced injuries on the antioxidant status and apoptosis-related gene expression in rats. J Toxicol Sci 36:285–296
Agarwal R, Goel SK, Behari JR (2010) Detoxification and antioxidant effects of curcumin in rats experimentally exposed to mercury. J Appl Toxicol 30:457–468
Aleo MF, Morandini F, Bettoni F, Tanganelli S, Vezzola A, Giuliani R, Steimberg N, Boniotti J, Bertasi B, Losio N, Apostoli P, Mazzoleni G (2002) In vitro study of the nephrotoxic mechanism of mercuric chloride. Med Lav 93:267–278
Asbaghian S, Shafaghat A, Zarea K, Kasimov F, Salimi F (2011) Comparison of volatile constituents, and antioxidant and antibacterial activities of the essential oils of Thymus caucasicus, T. kotschyanus and T. vulgaris. Nat Prod Commun 6:137–140
Avci G, Kupeli E, Eryavuz A, Yesilada E, Kucukkurt I (2006) Antihypercholesterolaemic and antioxidant activity assessment of some plants used as remedy in Turkish folk medicine. J Ethnopharmacol 107:418–423
Baytop T (1984) Therapy with medicinal plants in Turkey. Publication of Istanbul University, Istanbul, No. 2355, Faculty of pharmacy, No. 40
Bonacker D, Stoiber T, Wang M, Böhm KJ, Prots I, Unger E, Their R, Bolt HM, Dege GH (2004) Genotoxicity of inorganic mercury salts based on disturbed microtubule function. Arch Toxicol 78:575–583
Carmona ER, Kossatz E, Creus A, Marcos R (2008) Genotoxic evaluation of two mercury compounds in the Drosophila wing spot test. Chemosphere 70:1910–1914
Cinkilic N, Kiyici S, Celikler S, Vatan O, Gul OO, Tuncel E, Bilaloglu R (2009) Evaluation of chromosome aberrations, sister chromatid exchange and micronuclei in patients with type-1 diabetes mellitus. Mutat Res 676:1–4
Daneshvar-Royandezagh S, Khawar KM, Ozcan S (2009) In vitro micropropagation of garden thyme (Thymbra spicata L. var. Spicata L.) collected from Southeastern Turkey using cotyledon node. Biotechnol Biotechnol Eq 23:1319–1321
Datta S, Biswas SJ, Khuda-Bukhsh AR (2004) Comparative efficacy of pre-feeding, post- feeding and combined pre- and post-feeding of two microdoses of a potentized homeopathic drug, mercurius solubilis, in ameliorating genotoxic effects produced by mercuric chloride in mice. Evid Based Complem Altern Med 1:291–300
Dorman HJ, Bachmayer O, Kosar M, Hiltunen R (2004) Antioxidant properties of aqueous extracts from selected lamiaceae species grown in Turkey. J Agric Food Chem 52:762–770
El Babili F, Bouajila J, Souchard JP, Bertrand C, Bellvert F, Fouraste I, Moulis C, Valentin A (2011) Oregano: chemical analysis and evaluation of ıts antimalarial, antioxidant, and cytotoxic activities. J Food Sci 76:512–518
Evans HJ, O’Riordan ML (1975) Human peripheral blood lymphocytes for analysis of chromosome aberrations in mutagen tests. Mutat Res 31:135–148
Fenech M (1993) The cytokinesis blocks micronucleus technique: a detailed description on the method and its application to genotoxicity studies in human population. Mutat Res 285:35–44
Fitzgerald WF, Clarkson TW (1991) Mercury and monomethylmercury: present and future concerns. Environ Health Perspect 96:159–166
Fujiwara Y, Banno H, Shinkai Y, Yamamoto C, Kaji T, Satoh M (2011) Protective effect of pretreatment with cilostazol on cytotoxicity of cadmium and arsenite in cultured vascular endothelial cells. J Toxicol Sci 36:155–161
Glaser V, Nazari EM, Müller YM, Feksa L, Wannmacher CM, Rocha JB, de Bem AF, Farina M, Latini A (2010) Effects of inorganic selenium administration in methylmercury induced neurotoxicity in mouse cerebral cortex. Int J Dev Neurosci 28:631–637
Grotto D, Vicentini J, Angeli JP, Latorraca EF, Monteiro PA, Barcelos GR, Somacal S, Emanuelli T, Barbosa Jr F (2010) Evaluation of protective effects of fish oil against oxidative damage in rats exposed to methylmercury. Ecotoxicol Environ Saf. (doı:10.1016/j.ecoenv.2010.10.012) (in press)
Halder A, Patra M, De M (2005) Evaluation of mercury toxicity by some cytological indices in leucocyte cultures. Indian J Exp Biol 43:737–739
Haleagrahara N, Jackie T, Chakravarthi S, Rao M, Kulur A (2010) Protective effect of Etlingera elatior (torch ginger) extract on lead acetate–induced hepatotoxicity in rats. J Toxicol Sci 35:663–671
Jan AT, Ali A, Haq Q (2011) Glutathione as an antioxidant in inorganic mercury induced nephrotoxicity. J Postgrad Med (in press)
Kedziora K, Natowska K, Czuczejko J, Pawluk H, Kornatowski T, Motyl J, Szadujkis-Szadurski L, Szewczyk-Golec K, Kedziora J (2004) The markers of oxidative stress and activity of the antioxidant system in the blood of elderly patients with essential arterial hypertension. Cell Mol Biol Lett 9:635–641
Kiliç T (2006) Analysis of essential oil composition of Thymbra spicata var. spicata: antifungal, antibacterial and antimycobacterial activities. Z Naturforsch C 61:324–328
Lee KH, Abe S, Yanabe Y, Matsuda I, Yoshida MC (1990) Superoxide-dismutase activity and chromosome damage in cultured chromosome instability syndrome cells. Mutat Res 244:251–256
Rao MV, Chinoy NJ, Suthar MB, Rajvanshi MI (2001) Role of ascorbic acid on mercuric chloride-induced genotoxicity in human blood cultures. Toxicol In Vitro 15:649–654
Rao MV, Purohit A, Patel T (2010) Melatonin protection on mercury-exerted brain toxicity in the rat. Drug Chem Toxicol 33:209–216
Rozgaj R, Kasuba V, Blanusa M (2005) Mercury chloride genotoxicity in rats following oral exposure, evaluated by comet assay and micronucleus test. Arh Hig Rada Toksikol 56:9–15
Schurz F, Sabater-Vilar M, Fink-Gremmels J (2000) Mutagenicity of mercury chloride and mechanisms of cellular defense: the role of metal-binding proteins. Mutagenesis 15:525–530
Shelef LA (1983) Antimicrobial effects of spices. J Food Saf 6:29–44
Silva-Pereira LC, Cardoso PC, Leite DS, Bahia MO, Bastos WR, Smith MA, Burbano RR (2005) Cytotoxicity and genotoxicity of low doses of mercury chloride an methylmercury chloride on human lymphocytes in vitro. Braz J Med Biol Res 38:901–907
Takaishi M, Sawada M, Shimada A, Suzuki JS, Satoh M, Nagase H (2009) Protective role of metallothionein in benzo[a]pyrene-induced DNA damage. J Toxicol Sci 34:449–458
Therman E, Susman M (1993) Human chromosomes: structure, behaviour, and effects. J Med Genet 29:699–703
Toroğlu S, Dığrak M, Kocabaş YA (2005) Çay veya Baharat Olarak Tüketilen Teucrium polium L., Thymbra spicata L. var. spicata, Ocimum basilicum L. ve Foeniculum vulgare Miller’in Uçucu Yağlarının In Vitro Antimikrobiyal Aktivitesi ve Bazı Antibiyotiklerle Etkileşimleri. KSÜ Fen ve Mühendislik Dergisi 8:36–42
Tsangaris C, Vergolyas M, Fountoulaki E, Nizheradze K (2010) Oxidative stress and genotoxicity biomarker responses in grey mullet (Mugil cephalus) from a polluted environment in Saronikos Gulf, Greece. Arch Environ Contam Toxicol. (doi:10.1007/s00244-010-9629-8) (in press)
Turkez H, Dirican E (2011) A modulator against mercury chloride-induced genotoxic damage: Dermatocarpon intestiniforme (L.). Toxicol Ind Health. (doı:10.1177/0748233711404036) (in press)
Turkez H, Geyikoglu F (2010) Boric acid: a potential chemoprotective agent against aflatoxin b1 toxicity in human blood. Cytotechnology 62:157–165
Turkez H, Geyikoglu F, Aslan A, Karagöz Y, Turkez O, Anar M (2010) Antimutagenic effects of lichen Pseudovernia furfuracea (L.) Zoph. extracts against the mutagenicity of aflatoxin B1 in vitro. Toxicol Ind Health 26:625–631
Turkez H, Geyikoglu F, Tatar A, Keles MS, Kaplan I (2011) The effects of some boron compounds against heavy metal toxicity in human blood. Exp Toxicol Pathol. (doi:10.1016/j.etp.2010.06.011) (in press)
Unlü M, Vardar-Unlü G, Vural N, Dönmez E, Ozbaş ZY (2009) Chemical composition, antibacterial and antifungal activity of the essential oil of Thymbra spicata L. from Turkey. Nat Prod Res 23:572–579
Zalups RK, Koropatnick J (2000) Temporal changes in metallothionein gene transcription in rat kidney and liver: relationship to content of mercury and metallothionein protein. J Pharmacol Exp Ther 295:74–82
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Dirican, E., Turkez, H. & Toğar, B. Modulatory effects of Thymbra spicata L. different extracts against the mercury induced genotoxicity in human lymphocytes in vitro. Cytotechnology 64, 181–186 (2012). https://doi.org/10.1007/s10616-011-9406-1
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DOI: https://doi.org/10.1007/s10616-011-9406-1