Brief Report

Cytotechnology

, Volume 56, Issue 3, pp 145-149

First online:

A method for measuring mitochondrial mass and activity

  • Maria AgnelloAffiliated withDipartimento di Biologia Cellulare e dello Sviluppo “A. Monroy”, Università degli Studi di Palermo
  • , Giovanni MoriciAffiliated withDipartimento di Biologia Cellulare e dello Sviluppo “A. Monroy”, Università degli Studi di Palermo
  • , Anna Maria RinaldiAffiliated withDipartimento di Biologia Cellulare e dello Sviluppo “A. Monroy”, Università degli Studi di Palermo Email author 

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Abstract

Mitochondria, responsible for the energy-generating process essential for the cell metabolism, differ for the number, localization and activity in animal cells and tissues in relation to the energetic needs. Using fluorescent probes specific for mitochondria, Mitotracker Green (MTG) and Orange (MTO), and Confocal Laser-Scanning Microscope (CLSM), we elaborated a method to measure in vivo the mitochondrial mass and activity, in sea urchin Paracentrotus lividus eggs and embryos. The analysis of captured images, revealed a variation of mitochondrial distribution and an increase of activity after fertilization.

Keywords

CLSM Mitochondrial mass Mitochondrial activity MitoTracker Green MitoTracker Orange Sea urchin