Cytotechnology

, Volume 47, Issue 1, pp 51–57

Preparation of Cationic Immunovesicles Containing Cationic Peptide Lipid for Specific Drug Delivery to Target Cells

  • Takuya Sugahara
  • Shinichi Kawashima
  • Akiko Oda
  • Yoshio Hisaeda
  • Keiichi Kato
Article

DOI: 10.1007/s10616-005-3764-5

Cite this article as:
Sugahara, T., Kawashima, S., Oda, A. et al. Cytotechnology (2005) 47: 51. doi:10.1007/s10616-005-3764-5

Abstract

The cationic vesicle composed of Span80 and cationic peptide lipid (CPL) was prepared. The cytotoxicity of the Span80-CPL cationic vesicle was very low compared with Span80 vesicle. Antibody was able to be immobilized on vesicle surface by mediation of protein A. The antigen targeting ability of the antibody-immobilized vesicle (immunovesicle) derived from antibody was evaluated. Our results suggested that the Span80-CPL immunovesicles specifically associate with target cells by the antibody mediation, and the substance capsulated in immunovesicle was transferred into the target cells. This means that the Span80-CPL immunovesicle is expected to achieve a high local concentration of an encapsulated drug at the target.

Abbreviations

CPL

cationic peptide lipid

FBS

fetal bovine serum

FITC

fluorescein isothiocyanate

IADE

isothiocyanic acid dodecyl ester

PBS

phosphate buffered saline

PI

propidium iodide

Span80

sorbitan monooleate

Tween80

polyoxyethylene (20) sorbitan monooleate

Copyright information

© Springer 2005

Authors and Affiliations

  • Takuya Sugahara
    • 1
  • Shinichi Kawashima
    • 2
  • Akiko Oda
    • 1
  • Yoshio Hisaeda
    • 3
  • Keiichi Kato
    • 2
  1. 1.Faculty of AgricultureEhime UniversityMatsuyamaJapan
  2. 2.Faculty of EngineeringEhime UniversityMatsuyamaJapan
  3. 3.Faculty of EngineeringKyushu UniversityFukuokaJapan