Chemistry of Natural Compounds

, Volume 48, Issue 5, pp 906–907

Carotenoids from fruit of several Rosa species


    • Azerbaijan State Agrarian University
  • L. A. Shamsizade
    • Institute of BotanyNational Academy of Sciences

DOI: 10.1007/s10600-012-0420-0

Cite this article as:
Novruzov, A.R. & Shamsizade, L.A. Chem Nat Compd (2012) 48: 906. doi:10.1007/s10600-012-0420-0

Fruits of plants of the genus Rosa L. are rich in carotenoids [1]. They provide raw material for producing the preparation Carotolin and fatty oil [2]. In continuation of studies of Rosa species growing in Nakhchivan AR [3,4], we studied the quantitative content and qualitative composition of carotenoids from four Rosa species, R. canina L., R. corymbifera Borkh., R. nisami Sosn., and R. teberdensis Chrshan. section canina Crep.

Fruit samples for analysis were collected when ripe from various sites in the mountainous part of the republic, R. canina (Karabagly, Kengerli Region); R. corymbifera (Chalkhangala, Kengerli Region); R. nisami (Bichenak, Shakhbuz Region); R. teberdensis (Nurgut, Ordubad Region). Carotenoids were isolated by the method of Novruzov et al. [5]. The quantitative content of carotenoids was determined colorimetrically [6] on KFK-2. The carotenoid composition was established by TLC [7]. Absorption maxima were measured on a Specord-40 spectrophotometer.

The content and qualitative composition of carotenoids were established in freshly collected and dried fruit (the latter by sublimation). Total carotenoids from fresh (10 g) and dried (2 g) fruit were extracted exhaustively by n-hexane. In both instances, solvent was distilled off in vacuo. The residue was dissolved and saponified. Carotenoids were extracted by n-hexane. The hexane extract was worked up with H2O and dried over anhydrous Na2SO4 with subsequent evaporation in vacuo. Total carotenoids were dissolved in acetone (2 mL) containing the sodium salt of picric acid (0.01%, for internal standardization).

Pure carotenoids were isolated by TLC on silica gel on gypsum (LS, 5/40 mesh) using hexane:Et2O (17:3). Isolated carotenoids were identified by Rf value, color on the chromatogram, absorption maximum, and comparison with standards.

Table 1 presents results for the quantitative content and qualitative composition of carotenoids from fruit of the four Rosa L. species. It can be seen that the species differed in both content and qualitative composition. The carotenoid composition of R. canina, with the exception of poly-cis-lycopene A, was identical to that reported [810]. However, our results for the carotenoid composition of R. corymbifera fruit differed greatly from the literature. Carotenoids of R. corymbifera contained the known α- and β-carotene, lycopene, and ruboxanthin [11] in addition to poly-cis-lycopene, ε−carotene, cryptoxanthin, and taraxanthin.
Table 1

Quantitative Contents and Qualitative Composition of Carotenoids from Fruit of Rosa spp. Section Caninae


Content in fruit, mg%

Identified carotenoids



Rosa canina



Phytofluin, α-, β-carotene, poly-cis-lycopene A, lycopene, cryptoxanthin, ruboxanthin

R. corymbifera



α-, β-, ε-carotene, poly-cis-lycopene A, lycopene, cryptoxanthin, ruboxanthin, taraxanthin

R. nisami



β-, ε-carotene, poly-cis-lycopene A, lycopene, ruboxanthin, taraxanthin

R. teberdensis



α-, β-carotene, poly-cis-lycopene A, lycopene, cryptoxanthin, taraxanthin

The chromatographic data for the qualitative composition of carotenoids from fruit of Rosa species showed that they could be used as chemical signatures for establishing the species of polymorphic and ambiguous species.

Copyright information

© Springer Science+Business Media New York 2012