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Optimization of conditions for biocatalytic production of stigmast-4-en-3-one

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Pharmacologically active stigmast-4-en-3-one, the maximum formation of which (93.4 %) was achieved in the presence of n-hexadecane and palmitic acid, was produced by biotransformation of β-sitosterol (6 g/L) using rhodococcus cells. β-Sitosterol was added to the incubation medium as a mixture with β-cyclodextrin and Tween-80. The biotransformation processing conditions were optimized for β-sitosterol at high concentrations (10 g/L) using R. erythropolis IEGM 90 cells to catalyze formation of stigmast-4-en-3-one (75 %).

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Acknowledgment

The work was supported financially by the FTP “Research and Development in Priority Growth Areas of the Russian Science and Technology Complex for 2007–2012” (State Contract No. 16.518.11.7069) and the RFBR No. 10-04-96032-p_ural_a.

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Authors and Affiliations

  1. Institute of Technical Chemistry, Ural Branch, Russian Academy of Sciences, 614013, Russia, Perm, Ul. Akad. Koroleva, 3

    V. V. Grishko

  2. Institute of Ecology and Microorganism Genetics, Ural Branch, Russian Academy of Sciences, 614081, Russia, Perm, Ul. Goleva, 13

    E. M. Nogovitsina & I. B. Ivshina

Authors
  1. V. V. Grishko
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  2. E. M. Nogovitsina
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  3. I. B. Ivshina
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Correspondence to V. V. Grishko.

Additional information

Translated from Khimiya Prirodnykh Soedinenii, No. 3, May–June, 2012, pp. 390–392.

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Grishko, V.V., Nogovitsina, E.M. & Ivshina, I.B. Optimization of conditions for biocatalytic production of stigmast-4-en-3-one. Chem Nat Compd 48, 432–435 (2012). https://doi.org/10.1007/s10600-012-0267-4

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  • DOI: https://doi.org/10.1007/s10600-012-0267-4

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