Chemistry of Natural Compounds

, Volume 43, Issue 5, pp 519–524

Structure of lipid A from the marine gram-negative bacterium Pseudoalteromonas nigrifaciens IAM 13010T

  • A. S. Volk
  • I. N. Krasikova
  • S. D. Anastyuk
  • P. S. Dmitrenok
  • T. F. Solov’eva
Article

DOI: 10.1007/s10600-007-0181-3

Cite this article as:
Volk, A.S., Krasikova, I.N., Anastyuk, S.D. et al. Chem Nat Compd (2007) 43: 519. doi:10.1007/s10600-007-0181-3

Abstract

The structure of lipid A from the marine γ-proteobacterium Pseudoalteromonas nigrifaciens IAM 13010T that was prepared by hydrolysis of the corresponding lipopolysaccharide by acetic acid (1%) was determined by chemical analysis, 13C NMR spectroscopy, and MALDI/TOF and LSIMS mass spectrometry. It was shown that lipid A is a β-1,6-bonded disaccharide of glucosamine that is substituted by two phosphoric acids (in the C1 and C4′ positions), two (R)-3-hydroxyalkanoic (normal and branched) acids with ester bonding (at the C3 and C3′-positions), and (R)-3-hydroxydodecanoic and (R)-3-dodecanoyloxydodecanoic acids (both with amide bonding at C2′ and C2, respectively). It was hypothesized that this type of structure is typical of lipid A from bacteria of the genus Pseudoalteromonas in general.

Key words

marine gram-negative bacteriaPseudoalteromonas nigrifaciensLPSlipid A13C NMR spectroscopyMALDI/TOF and LSIMS mass spectrometry

Copyright information

© Springer Science+Business Media, Inc. 2007

Authors and Affiliations

  • A. S. Volk
    • 1
  • I. N. Krasikova
    • 1
  • S. D. Anastyuk
    • 1
  • P. S. Dmitrenok
    • 1
  • T. F. Solov’eva
    • 1
  1. 1.Pacific Institute of Bioorganic Chemistry, Far-East DivisionRussian Academy of SciencesVladivostok