Conservation Genetics

, Volume 14, Issue 4, pp 809–821

Microsatellite markers reveal clear geographic structuring among threatened noble crayfish (Astacus astacus) populations in Northern and Central Europe

  • Riho Gross
  • Stefan Palm
  • Kuldar Kõiv
  • Tore Prestegaard
  • Japo Jussila
  • Tiit Paaver
  • Juergen Geist
  • Harri Kokko
  • Anna Karjalainen
  • Lennart Edsman
Research Article

DOI: 10.1007/s10592-013-0476-9

Cite this article as:
Gross, R., Palm, S., Kõiv, K. et al. Conserv Genet (2013) 14: 809. doi:10.1007/s10592-013-0476-9

Abstract

Noble crayfish (Astacus astacus L.), the most highly valued freshwater crayfish in Europe, is threatened due to a long-term population decline caused mainly by the spread of crayfish plague. Reintroduction of the noble crayfish into restored waters is a common practice but the geographic and genetic origin of stocking material has rarely been considered, partially because previous genetic studies have been hampered by lack of nuclear gene markers with known inheritance. This study represents the first large scale population genetic survey of the noble crayfish (633 adults from 18 locations) based on 10 newly developed microsatellite markers. We focused primarily on the Baltic Sea area (Estonia, Finland and Sweden) where the largest proportion of the remaining populations exists. To allow comparisons, samples from the Black Sea catchment (the Danube drainage) were also included. Two highly differentiated population groups were identified corresponding to the Baltic Sea and the Black Sea catchments, respectively. The Baltic Sea catchment populations had significantly lower genetic variation and private allele numbers than the Black Sea catchment populations. Within the Baltic Sea area, a clear genetic structure was revealed with population samples corresponding well to their geographic origin, suggesting little impact of long-distance translocations. The clear genetic structure strongly suggests that the choice of stocking material for re-introductions and supplemental releases needs to be based on empirical genetic knowledge.

Keywords

Genetic variationGenetic differentiationPopulation structureMicrosatellite DNAConservation genetics

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Riho Gross
    • 1
  • Stefan Palm
    • 2
  • Kuldar Kõiv
    • 1
  • Tore Prestegaard
    • 2
  • Japo Jussila
    • 3
  • Tiit Paaver
    • 1
  • Juergen Geist
    • 4
  • Harri Kokko
    • 3
  • Anna Karjalainen
    • 3
  • Lennart Edsman
    • 2
  1. 1.Department of AquacultureInstitute of Veterinary Medicine and Animal Sciences, Estonian University of Life SciencesTartuEstonia
  2. 2.Department of Aquatic ResourcesInstitute of Freshwater Research, Swedish University of Agricultural SciencesDrottningholmSweden
  3. 3.Department of BiologyUniversity of Eastern FinlandKuopioFinland
  4. 4.Aquatic Systems Biology Unit, Department of Ecology and Ecosystem ManagementTechnische Universität München FreisingGermany