Abstract
The scaly-sided merganser, Mergus squamatus, is considered one of the most threatened sea duck species in the Palearctic with limited breeding and wintering distribution in China and Russia. To provide information for future conservation efforts, we sequenced a portion of the mitochondrial (mt) DNA control region in four species of mergansers and three additional sea duck taxa to characterize the evolutionary history of the scaly-sided merganser, infer population trends that may have led to its limited geographic distribution, and to compare indices of genetic diversity among species of mergansers. Scaly-sided mergansers exhibit substantially lower levels of mtDNA genetic diversity (h = 0.292, π = 0.0007) than other closely related sea ducks and many other avian taxa. The four haplotypes observed differed by a single base pair suggesting that the species has not experienced a recent population decline but has instead been at a low population level for some time. A phylogenetic analysis placed the scaly-sided merganser basal to North American and European forms of the common merganser, M. merganser. Our inclusion of a small number of male samples doubled the number of mtDNA haplotypes observed, suggesting that additional genetic variation likely exists within the global population if there is immigration of males from unsampled breeding areas.
References
Bollinger KS, Derksen DV (1996) Demographic characteristics of molting black brant at Teshekpuk Lake, Alaska. J Field Ornithol 67:141–158
Collar NJ, Crosby MJ, Stattersfield AJ (1994) Birds to watch 2: the world checklist of threatened birds. BirdLife International, Cambridge
Drummond AJ, Rambaut A (2007) BEAST: Bayesian evolutionary analysis by sampling trees. BMC Evol Biol 7:214
Excoffier L, Laval G, Schneider S (2005) ARLEQUIN version 3.1: an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50
Flint PL, Petersen MR, Dau CP, Hines JE, Nichols JD (2000) Annual survival and site fidelity of Steller’s eiders molting along the Alaska Peninsula. J Wild Manage 64:261–268
Fu YX (1997) Statistical tests on neutrality of mutations against population growth, hitchhiking and background selection. Genetics 147:915–925
He F-Q, Melville D, Gui X-J, Hong Y-H, Liu Z-Y (2002) Status of the scaly-sided merganser wintering in mainland China in the 1990’s. Waterbirds 25:462–464
Hefti-Gautschi B, Pfunder M, Jenni L, Keller V, Ellegren H (2009) Identification of conservation units in the European Mergus merganser based on nuclear and mitochondrial DNA markers. Conserv Genet 10:87–99
Iliashenko VY, Iliashenko EI (2000) Krasnaya kniga Rossii: pravovye akty [Red data book of Russia: legislative acts]. State committee of the Russian Federation for Environmental Protection, Moscow, p 143 (in Russian)
IUCN (International Union for Conservation of Nature and Natural Resources) (2008) 2008 IUCN Red List of Threatened Species. http://www.iucnredlist.org. Cited 22 June 2010
Kear J (2005) Ducks, geese and swans, volume 2. Oxford University Press, New York
Kuro-o M, Yonekawa H, Saito S, Eda M, Higuchi H, Koike H, Hasegawa H (2010) Unexpectedly high genetic diversity of mtDNA control region through severe bottleneck in vulnerable albatross Phoebastria albatrus. Conserv Genet 11:127–137
Liu P, Li F, Song H, Wang Q, Song Y, Liu Y, Zh Piao (2010) A survey to the distribution of the scaly-sided Merganser (Mergus squamatus) in Changbai mountain range (China side). Chin Birds 1(2):148–155
Livezey BC (1995) Phylogeny and evolutionary ecology of modern seaducks (Anatidae: Mergini). Condor 97:233–255
McCusker MR, Bentzen P (2010) Positive relationships between genetic diversity and abundance in fishes. Mol Ecol 19:4852–4862
Miller MJ, Bermingham E, Klicka J, Escalante P, Winder K (2010) Neotropical birds show a humped distribution of within-population genetic diversity along a latitudinal transect. Ecol Lett 13:576–586
Pearce JM, Petersen MR (2009) Post-fledging movements of juvenile common mergansers (Mergus merganser) in Alaska as inferred by satellite telemetry. Waterbirds 32:133–137
Pearce JM, Talbot SL, Petersen MR, Rearick JR (2005) Limited genetic differentiation among breeding, molting, and wintering groups of the threatened Steller’s eider: the role of historic and contemporary factors. Conserv Genet 6:743–757
Pearce JM, Blums P, Lindberg M (2008) Site fidelity is an inconsistent determinant of population structure in the hooded merganser (Lophodytes cucullatus): evidence from genetic, mark-recapture, and comparative data. Auk 125:711–722
Pearce JM, Zwiefelhofer D, Maryanski N (2009a) Mechanisms of population heterogeneity among molting common mergansers on Kodiak Island, Alaska: implications for genetic assessments of migratory connectivity. Condor 111:283–293
Pearce JM, McCracken KG, Christensen TK, Zhuravlev YN (2009b) Migratory patterns and population structure among breeding and wintering red-breasted mergansers (Mergus serrator) and common mergansers (M. merganser). Auk 126:784–798
Peters JL, McCracken KG, Zhuravlev YN, Lu Y, Wilson RE, Johnson KP, Omland KE (2005) Phylogenetics of wigeons and allies (Anatidae: Anas): the importance of sampling multiple loci and multiple individuals. Mol Phylogenet Evol 35:209–224
Rogers AR, Harpending HC (1992) Population growth makes waves in the distribution of pairwise genetic differences. Mol Biol Evol 9:552–569
Ruokonen M, Asrvak T, Chesser RK, Lundqvist AC, Merilaä J (2010) Temporal increase in mtDNA diversity in a declining population. Mol Ecol 19:2408–2417
Scribner KT, Petersen MR, Fields RL, Talbot SL, Pearce JM, Chesser RK (2001) Sex-biased gene flow in spectacled eiders (Anatidae): inferences from molecular markers with contrasting modes of inheritance. Evolution 55:2105–2115
Solovieva D, Shokhrin V, Vartanyan S, Dondua A, Vartanyan N (2006) Scaly-sided Merganser surveys in Primorye, Russia, 2003–05. Threat Waterfowl Specialist Group News 15:60–69
Surmach SG, Zaykin DV (1994) The scaly-sided Merganser Mergus squamatus (Gould) in the Iman basin, Far-East Russia. In: Hughes B and Hunter J (eds) The scaly-sided Merganser Mergus squamatus in Russia and China. Special publication of the Wildfowl and Wetlands Trust No. 1, pp 11–17
Tajima F (1989) The amount of DNA polymorphism maintained in a finite population when the neutral mutation rate varies among sites. Genetics 143:1457–1465
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
Villesen P (2007) FaBox: an online toolbox for FASTA sequences. Mol Ecol Res 7:965–968
Zhao Z, Han X, Zhang S, Wu J (1995) Breeding ecology of the Chinese merganser in the Changbai mountains, China. J Field Ornithol 66:54–59
Zink RM, Remsen JV Jr (1986) Evolutionary processes and patterns of geographic variation in birds. In: Johnston RF (ed) Current ornithology no. 4. Plenum Press, New York, pp 1–70
Acknowledgments
Field work was supported through the Wildfowl and Wetlands Trust, the Rufford Small Grant Program, and the Forestry Bureau COA of Taiwan. A. Ramey, D. Derksen, and M. Kuro-o provided comments on earlier drafts of the manuscript. Any use of trade, product, or firm names in this publication is for descriptive purposes only and does not imply endorsement by the US Government.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Solovyeva, D.V., Pearce, J.M. Comparative mitochondrial genetics of North American and Eurasian mergansers with an emphasis on the endangered scaly-sided merganser (Mergus squamatus). Conserv Genet 12, 839–844 (2011). https://doi.org/10.1007/s10592-010-0180-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-010-0180-y