Conservation Genetics

, Volume 11, Issue 5, pp 1911–1928

Microsatellite markers and mtDNA data indicate two distinct groups in dwarf galaxias, Galaxiella pusilla (Mack) (Pisces: Galaxiidae), a threatened freshwater fish from south-eastern Australia

  • R. A. Coleman
  • V. Pettigrove
  • T. A. Raadik
  • A. A. Hoffmann
  • A. D. Miller
  • M. E. Carew
Research Article

DOI: 10.1007/s10592-010-0082-z

Cite this article as:
Coleman, R.A., Pettigrove, V., Raadik, T.A. et al. Conserv Genet (2010) 11: 1911. doi:10.1007/s10592-010-0082-z

Abstract

Galaxiella pusilla is a small, non-migratory freshwater fish, endemic to south-eastern Australia and considered nationally threatened. To assist in the conservation of the species, microsatellite markers were developed and used to characterize genetic variation in 20 geographically distinct populations across its range. Substantial genetic differentiation was found between an eastern (Victoria east of the Otway Ranges and Tasmania) and western (South Australia and Victoria west of, and including, the Otway Ranges) region. This major separation was also observed in data from a mitochondrial gene and supports a previously proposed split. Populations from the eastern region had overall lower genetic diversity for both the microsatellite and mtDNA markers. There was substantial genetic differentiation between populations within the two regions, suggesting that gene flow is limited by the isolation of freshwater streams. Genetic structure, consistent with an isolation-by-distance model, was also evident in both regions. Patterns of genetic variation in this threatened species are compared to those obtained for other taxa across the same region. The need to consider separate conservation strategies for the two sets of populations is emphasized.

Keywords

Galaxiella pusillaThreatened speciesFreshwater fishPopulation geneticsMicrosatellitesCOI

Supplementary material

10592_2010_82_MOESM1_ESM.doc (338 kb)
Supplementary material 1 (DOC 339 kb)

Copyright information

© Springer Science+Business Media B.V. 2010

Authors and Affiliations

  • R. A. Coleman
    • 1
    • 2
  • V. Pettigrove
    • 1
    • 2
  • T. A. Raadik
    • 3
  • A. A. Hoffmann
    • 1
  • A. D. Miller
    • 1
  • M. E. Carew
    • 1
  1. 1.Centre for Environmental Stress and Adaptation Research, Department of Zoology and GeneticsThe University of MelbourneParkvilleAustralia
  2. 2.Melbourne Water CorporationMelbourneAustralia
  3. 3.Department of Sustainability and EnvironmentArthur Rylah Institute for Environmental ResearchHeidelbergAustralia