Technical Note

Conservation Genetics

, Volume 11, Issue 3, pp 1193-1195

First online:

Isolation and characterization of 14 polymorphic microsatellite loci in the ringneck snake Diadophis punctatus (Colubridae: Dipsadinae)

  • Frank M. FontanellaAffiliated withThe Graduate Center, City University of New York Email author 
  • , Mark E. SiddallAffiliated withSackler Institute of Comparative Genomics, American Museum of Natural History

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We constructed a genomic library and developed 14 polymorphic loci to assess species boundaries between previously described mtDNA lineages and to examine the genetic variability between populations of the ringneck snake Diadophis punctatus species complex. Polymorphism was investigated for these markers in 34 individuals from populations in southeastern Louisiana and southern Florida. The number of alleles ranged from 5 to 29, expected heterozygosity ranged from 0.816 to 0.962 and observed heterozygosity ranged from 0.452 to 0.889. These new loci provide the tools necessary to examine species boundaries, population genetics within species and to determine the conservation status of imperiled populations.


Microsatellites Heterozygosity Species boundaries Diadophis punctatus