Technical Note

Conservation Genetics

, Volume 10, Issue 5, pp 1515-1519

Development of microsatellite DNA markers of grass carp (Ctenopharyngodon idella) and their cross-species application in black carp (Mylopharyngodon piceus)

  • Shanshan GuoAffiliated withCollege of Marine Life Sciences, Ocean University of China
  • , Guiwei ZouAffiliated withYangtze River Fisheries Institute, Chinese Academy of Fishery
  • , Guanpin YangAffiliated withCollege of Marine Life Sciences, Ocean University of China Email author 

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Abstract

Thirty-four microsatellite DNA loci were developed for grass carp (Ctenopharyngodon idella) and used to determine the diversity of 42 individuals of grass carp and 16 individuals of black carp collected from Jingzhou fragment (the middle reach) of Yangtze River. All the 34 loci were polymorphic in grass carp. The number of alleles found in grass carp at these loci ranged from 2 to 24. The observed and expected heterozygosities varied from 0.238 to 1.000 and from 0.162 to 0.945, respectively. Thirty of the 34 loci cross-amplified the DNA of black carp (Mylopharyngodon piceus), and 21 of them revealed polymorphism (more than one allele each locus).

Keywords

Grass carp Ctenopharyngodon idella Black carp Mylopharyngodon piceus Microsatellite DNA Polymorphism