Conservation Genetics

, Volume 10, Issue 1, pp 225–236

Efficiency of markers and methods for detecting hybrids and introgression in stocked populations


    • Laboratori d’Ictiologia GenèticaUniversitat de Girona
  • Rosa M. Araguas
    • Laboratori d’Ictiologia GenèticaUniversitat de Girona
  • Raquel Fernández
    • Laboratori d’Ictiologia GenèticaUniversitat de Girona
  • Manuel Vera
    • Laboratori d’Ictiologia GenèticaUniversitat de Girona
  • José-Luis García-Marín
    • Laboratori d’Ictiologia GenèticaUniversitat de Girona
Research Article

DOI: 10.1007/s10592-008-9550-0

Cite this article as:
Sanz, N., Araguas, R.M., Fernández, R. et al. Conserv Genet (2009) 10: 225. doi:10.1007/s10592-008-9550-0


Detection of hybridization and introgression in wild populations that have been supplemented by hatchery fish is necessary during development of conservation and management strategies. Initially, allozyme data and more recently highly polymorphic microsatellite markers have been used to obtain this information. We used both markers to assess the effectiveness of four assignment methods (Structure, NewHybrids, Baps and GeneClass) to detect hatchery introgression in wild stocked populations. Simulations of hybrid genotypes from real parental data revealed that the number and type of markers used with Structure, NewHybrids and Baps can identify as admixed most first and second generation hybrids as well as first generation backcrosses. In wild populations, introgression rates estimated from different markers and methods were correlated. However, slight disagreements were observed at both population and individual levels. Overall, the fully Bayesian (Structure, NewHybrids and Baps) performed better than partially Bayesian (GeneClass) assignment tests. In wild collections, Baps analyses were limited because of the lack of a native baseline. In all cases, the efficiency of methods was reduced as introgression increased.


AllozymesAssignment methodsBrown troutIntrogressionMicrosatellites

Copyright information

© Springer Science+Business Media B.V. 2008