Conservation Genetics

, Volume 9, Issue 5, pp 1395–1398

Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.)

  • Madjid Delghandi
  • Mette Serine Wesmajervi
  • Saskia Mennen
  • Frank Nilsen
Technical Note

DOI: 10.1007/s10592-008-9507-3

Cite this article as:
Delghandi, M., Wesmajervi, M.S., Mennen, S. et al. Conserv Genet (2008) 9: 1395. doi:10.1007/s10592-008-9507-3
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Abstract

Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy–Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.

Keywords

Atlantic cod Gadus morhua cDNA Expressed sequence tags (ESTs) Microsatellites Type I markers 

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Madjid Delghandi
    • 1
    • 2
  • Mette Serine Wesmajervi
    • 1
  • Saskia Mennen
    • 1
  • Frank Nilsen
    • 3
  1. 1.Norwegian Institute of Fisheries and Aquaculture ResearchTromsoNorway
  2. 2.Department of Marine Science and FisheriesCollege of Agricultural and Marine Sciences, Sultan Qaboos UniversityMuscatSultanate of Oman
  3. 3.University of Bergen, Institute of BiologyBergenNorway

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