Technical Note

Conservation Genetics

, Volume 9, Issue 5, pp 1395-1398

First online:

Development of twenty sequence-tagged microsatellites for the Atlantic cod (Gadus morhua L.)

  • Madjid DelghandiAffiliated withNorwegian Institute of Fisheries and Aquaculture ResearchDepartment of Marine Science and Fisheries, College of Agricultural and Marine Sciences, Sultan Qaboos University Email author 
  • , Mette Serine WesmajerviAffiliated withNorwegian Institute of Fisheries and Aquaculture Research
  • , Saskia MennenAffiliated withNorwegian Institute of Fisheries and Aquaculture Research
  • , Frank NilsenAffiliated withUniversity of Bergen, Institute of Biology

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Fifty-four primer pairs were designed for expressed sequence tag (EST) sequences containing perfect di- and tri-nucleotide motifs and characterised in 96 unrelated fish. Twenty markers were successfully amplified with number of alleles from 2 to 10 per locus and observed and expected heterozygosity ranging from 0.01 to 0.56 and 0.03 to 0.70, respectively. Loci Gmo-C213, Gmo-C246 and Gmo-C247 deviated from Hardy–Weinberg equilibrium. Genetic linkage disequilibrium analysis between all pairs of the loci showed significant departure from the null hypothesis between loci Gmo-C213 and Gmo-C222, Gmo-C233 and Gmo-C229, C223 and Gmo-C236 and C229 and Gmo-C236. The gene identity was determined at 10 of the loci, confirming the associated microsatellites as Type I markers. These microsatellite markers provide useful tools for studies of population genetics, reproductive ecology and constructing linkage maps of Atlantic cod.


Atlantic cod Gadus morhua cDNA Expressed sequence tags (ESTs) Microsatellites Type I markers