Conservation Genetics

, Volume 9, Issue 5, pp 1385–1388

Development and characterization of microsatellite loci for lotus (Nelumbonucifera)

  • Hong-Li Tian
  • Xiao-Qing Chen
  • Jian-Xiu Wang
  • Jian-Hua Xue
  • Jun Wen
  • Grant Mitchell
  • Shi-Liang Zhou
Technical Note

DOI: 10.1007/s10592-007-9503-z

Cite this article as:
Tian, HL., Chen, XQ., Wang, JX. et al. Conserv Genet (2008) 9: 1385. doi:10.1007/s10592-007-9503-z

Abstract

This paper reports the development of microsatellite primers for Nelumbo nucifera Gaerten. By screening genomic libraries enriched with 10 kinds of probes, Seventeen polymorphic loci were isolated and primers were designed. Polymorphism of these 17 loci was assessed in 24 individuals. All the 17 loci are polymorphic and the number of alleles ranged from two to seven. Observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.9176 and from 0.2837 to 0.7917 respectively. These microsatellite loci should be useful for studying the genetic diversity of N. nucifera.

Keywords

Nelumbo nuciferaGenetic diversityMicrosatellite primersSSR

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Hong-Li Tian
    • 1
    • 2
  • Xiao-Qing Chen
    • 1
    • 2
  • Jian-Xiu Wang
    • 1
    • 2
  • Jian-Hua Xue
    • 3
  • Jun Wen
    • 1
    • 4
  • Grant Mitchell
    • 5
  • Shi-Liang Zhou
    • 1
  1. 1.State Key Laboratory of Systematic and Evolutionary Botany, Institute of BotanyChinese Academy of SciencesBeijingChina
  2. 2.Graduate School of the Chinese Academy of SciencesBeijingChina
  3. 3.Department of Life SciencesDaqing Normal CollegeDaqingChina
  4. 4.Department of BotanyNational Museum of Natural History, Smithsonian InstitutionWashingtonUSA
  5. 5.Liang Lian Lotus & Waterlily FarmBungaAustralia