Technical Note

Conservation Genetics

, Volume 9, Issue 4, pp 1051-1054

First online:

Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica

  • Angélica Cibrián-JaramilloAffiliated withGenomics, The New York Botanical GardenSackler Institute for Comparative Genomics, American Museum of Natural History Email author 
  • , Thomas E. MarlerAffiliated withWestern Pacific Tropical Research Center, College of Natural and Applied Sciences, University of Guam
  • , Rob DeSalleAffiliated withSackler Institute for Comparative Genomics, American Museum of Natural History
  • , Eric D. BrennerAffiliated withGenomics, The New York Botanical Garden

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We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform conservation efforts and disentangle the biogeographic history of the genus.


Cycad Cycas micronesica EST-STRs Invasives