Conservation Genetics

, Volume 9, Issue 4, pp 1051–1054

Development of EST-microsatellites from the cycad Cycas rumphii, and their use in the recently endangered Cycas micronesica

  • Angélica Cibrián-Jaramillo
  • Thomas E. Marler
  • Rob DeSalle
  • Eric D. Brenner
Technical Note

DOI: 10.1007/s10592-007-9447-3

Cite this article as:
Cibrián-Jaramillo, A., Marler, T.E., DeSalle, R. et al. Conserv Genet (2008) 9: 1051. doi:10.1007/s10592-007-9447-3

Abstract

We developed a set of 12 EST-microsatellite markers (EST-STRs) from Cycas rumphii Miq. and tested them on an ex situ collection of the endangered Cycas micronesica K.D. Hill. The number of alleles per locus in both species ranged from 1 to 15. Observed and expected heterozygosities ranged from 0.000 to 0.842, and 0.000 to 0.883, respectively. All primers amplified in four additional Cycas species. These markers are the first genome-enabled tools for cycad population level studies, and are now available to inform conservation efforts and disentangle the biogeographic history of the genus.

Keywords

CycadCycas micronesicaEST-STRsInvasives

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Angélica Cibrián-Jaramillo
    • 1
    • 2
  • Thomas E. Marler
    • 3
  • Rob DeSalle
    • 2
  • Eric D. Brenner
    • 1
  1. 1.GenomicsThe New York Botanical Garden BronxUSA
  2. 2.Sackler Institute for Comparative GenomicsAmerican Museum of Natural HistoryNew YorkUSA
  3. 3.Western Pacific Tropical Research Center, College of Natural and Applied Sciences University of GuamGuamUSA