Technical Note

Conservation Genetics

, Volume 8, Issue 6, pp 1495-1497

First online:

Development and characterization of microsatellite markers for an endangered shrub, Ammopiptanthus mongolicus (Leguminosae) and cross-species amplification in Ammopiptanthus nanus

  • Guo-Qing ChenAffiliated withSouth China Botanical Garden, The Chinese Academy of SciencesGraduate School of the Chinese Academy of Sciences
  • , Hong-Wen HuangAffiliated withSouth China Botanical Garden, The Chinese Academy of SciencesWuhan Botanical Garden, The Chinese Academy of Sciences
  • , Ming KangAffiliated withWuhan Botanical Garden, The Chinese Academy of Sciences
  • , Xue-Jun GeAffiliated withState Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University Email author 

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Abstract

We developed 11 polymorphic microsatellite markers for an endangered shrub Ammopiptanthus mongolicus using the Fast Isolation by AFLP of Sequences Containing repeats protocol. Polymorphism of each locus was assessed in 24 individuals from five natural populations of A. mongolicus. The average allele number of these markers was 4.2, ranging from 2 to 7. The observed (H O) and expected (H E) heterozygosities were 0.044–0.956 and 0.044–0.827, respectively. Further assessment in the congeneric species A. nanus revealed that nine of eleven loci were successfully amplified, but only two loci showed polymorphism. These markers provide powerful tools for the conservation genetics studies of A. mongolicus.

Keywords

Ammopiptanthus mongolicus Endangered species FIASCO Genetic diversity Microsatellite markers