Conservation Genetics

, Volume 9, Issue 1, pp 225–228

PCR-CTPP: a rapid and reliable genotyping technique based on ZFX/ZFY alleles for sex identification of tiger (Panthera tigris) and four other endangered felids

Authors

  • Kun Wei
    • Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life SciencesSichuan University
  • Zhihe Zhang
    • Key Laboratory of Endangered Wildlife Breeding and Conservation Genetics of Sichuan ProvinceChengdu Research Base of Giant Panda Breeding
  • Wenping Zhang
    • Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life SciencesSichuan University
    • College of ScienceHonghe University
  • Xiao Xu
    • Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life SciencesSichuan University
  • Xu Liang
    • Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life SciencesSichuan University
  • Guangxin He
    • Key Laboratory of Endangered Wildlife Breeding and Conservation Genetics of Sichuan ProvinceChengdu Research Base of Giant Panda Breeding
  • Fujun Shen
    • Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life SciencesSichuan University
    • Key Laboratory of Endangered Wildlife Breeding and Conservation Genetics of Sichuan ProvinceChengdu Research Base of Giant Panda Breeding
  • Liang Zhang
    • Key Laboratory of Endangered Wildlife Breeding and Conservation Genetics of Sichuan ProvinceChengdu Research Base of Giant Panda Breeding
  • Rong Hou
    • Key Laboratory of Endangered Wildlife Breeding and Conservation Genetics of Sichuan ProvinceChengdu Research Base of Giant Panda Breeding
    • Key Laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life SciencesSichuan University
Short Communication

DOI: 10.1007/s10592-006-9279-6

Cite this article as:
Wei, K., Zhang, Z., Zhang, W. et al. Conserv Genet (2008) 9: 225. doi:10.1007/s10592-006-9279-6

Abstract

An inexpensive, time-saving and reliable method, polymerase chain reaction with confronting two-pair primers (PCR-CTPP), was developed for sex identification in tiger (Panthera tigris) based on zinc finger alleles (ZFX/ZFY). A site of “C/G” transversion representing fixed differences that discriminated between ZFX and ZFY exons among felids was identified for primers designing. This primer set was successfully tested on samples including blood, shed hairs, dried skin, and stool which contained potential contamination caused by prey DNA. Cross species tests shown that this primer set was also useful for sex identification in four other endangered felids.

Keywords

Felids PCR-CTPP Sex identification Tiger ZFX ZFY

Copyright information

© Springer Science+Business Media, Inc. 2007