Conservation Genetics

, Volume 8, Issue 5, pp 1263–1266

Isolation and characterization of microsatellites in Primula glaucescens Moretti and their cross-species amplification in P. spectabilis Tratt.

Authors

    • Dipartimento di Biotecnologie e Scienze Molecolari (DBSM)Università degli Studi dell’Insubria
    • School of Biology & Psychology, Division of BiologyUniversity of Newcastle upon Tyne
    • Dipartimento di Scienze Biomolecolari e Biotecnologie (DSBB)Università degli Studi di Milano
  • Marilena Meloni
    • Dipartimento di Biotecnologie e Scienze Molecolari (DBSM)Università degli Studi dell’Insubria
  • Kirsten Wolff
    • School of Biology & Psychology, Division of BiologyUniversity of Newcastle upon Tyne
  • Giorgio Binelli
    • Dipartimento di Biotecnologie e Scienze Molecolari (DBSM)Università degli Studi dell’Insubria
Technical note

DOI: 10.1007/s10592-006-9258-y

Cite this article as:
Perini, D., Meloni, M., Wolff, K. et al. Conserv Genet (2007) 8: 1263. doi:10.1007/s10592-006-9258-y

Abstract

The hexaploid herbaceous species Primula glaucescens Moretti and Primula spectabilis Tratt. (2= 6= 66) are two endemics of the Italian Pre-Alps protected by national and international laws. In order to plan conservation strategies for natural populations and to study the influence of the latest glaciation on them we developed a set of microsatellites markers for the endangered Primula species: ten primer pairs allowed analysis of polymorphic disomic loci in P. glaucescens samples and seven of them also amplified polymorphic disomic markers in P. spectabilis.

Keywords

Conservation and repopulation Microsatellite enriched library Hexaploid alpine plant species Primula glaucescens Moretti Primula spectabilis Tratt.

Copyright information

© Springer Science+Business Media, Inc. 2007