Conservation Genetics

, 8:1247

Genetic evidence of Ranavirus in toe clips: an alternative to lethal sampling methods

Technical Note

DOI: 10.1007/s10592-006-9242-6

Cite this article as:
St-Amour, V. & Lesbarrères, D. Conserv Genet (2007) 8: 1247. doi:10.1007/s10592-006-9242-6


Amphibian populations have been undergoing declines on a global scale. Among the many threats to these populations are emergent infectious diseases (EIDs). The Ranavirus in particular has been found within many declining amphibian populations. Although non-lethal sampling methods exist for some amphibian groups, such as salamanders, the anurans are traditionally tested using a lethal method. By comparing traditional liver samples and a new non-lethal method of toe clipping we prove that the Ranavirus can also be determined in frogs using a non-lethal method, a much needed tool in threatened populations. This method will allow for ranaviral detection without further impacting declining populations, and can further be used for other research questions.


RanavirusNon-invasive methodAmphibian declinesRana clamitans

Copyright information

© Springer Science+Business Media, Inc. 2006

Authors and Affiliations

  1. 1.Biology DepartmentLaurentian UniversitySudburyCanada