Technical Note

Conservation Genetics

, Volume 8, Issue 3, pp 757-759

A PCR-RFLP method on faecal samples to distinguish Martes martes, Martes foina, Mustela putorius and Vulpes vulpes

  • Lucentini LiviaAffiliated withDipartimento di Biologia Cellulare e Ambientale, Università degli Studi di Perugia Email author 
  • , Vercillo FrancescaAffiliated withDipartimento di Biologia Cellulare e Ambientale, Università degli Studi di Perugia
  • , Palomba AntonellaAffiliated withDipartimento di Biologia Cellulare e Ambientale, Università degli Studi di Perugia
  • , Panara FaustoAffiliated withDipartimento di Biologia Cellulare e Ambientale, Università degli Studi di Perugia
  • , Ragni BernardinoAffiliated withDipartimento di Biologia Cellulare e Ambientale, Università degli Studi di Perugia

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

A non-invasive genetic approach has been recently employed in the population genetics of wild species, using faeces that could be easily collected, particularly of elusive or endangered species. However, faeces of pine (Martes martes) and beech marten (M. foina) can be morphologically similar and could be confused with those of polecat (Mustela putorius) and red fox (Vulpes vulpes). On this basis, a rapid, simple and inexpensive RFLP protocol using a cytochrome b (Cyt b) gene fragment of mtDNA, isolated from faecal samples, was performed to distinguish the above-mentioned species.

Keywords

Mustelids Non-invasive sampling Conservation genetics Faeces analysis PCR-RFLP