Conservation Genetics

, Volume 8, Issue 3, pp 715–720

A reliable genetic technique for sex determination of giant panda (Ailuropoda melanoleuca) from non-invasively collected hair samples

  • Matthew E. Durnin
  • Per J. Palsbøll
  • Oliver A. Ryder
  • Dale R. McCullough
SHORT COMMUNICATION

DOI: 10.1007/s10592-006-9196-8

Cite this article as:
Durnin, M.E., Palsbøll, P.J., Ryder, O.A. et al. Conserv Genet (2007) 8: 715. doi:10.1007/s10592-006-9196-8

Abstract

Extractions from non-invasive hair samples usually yield low amounts of highly degraded DNA. Previously developed mammal molecular sexing methods were not designed with such sub-optimal conditions in mind. We developed a simple and reliable PCR-based sexing method aimed at degraded, low yield DNA extractions from the giant panda (Ailuropoda melanoleuca). Comparisons of this new primer set with others showed that the reliability of sex determination from low-yield, degraded DNA extractions was improved if; amplification products were short (<170 bp); and the Y-chromosome amplification product was shorter than the X-chromosome amplification product. The primers developed in this study appear useful for sex determination in other bear species.

Keywords

ZFXZFYSexUrsidaeBearPanda

Copyright information

© Springer Science+Business Media B.V. 2006

Authors and Affiliations

  • Matthew E. Durnin
    • 1
    • 3
  • Per J. Palsbøll
    • 1
  • Oliver A. Ryder
    • 2
  • Dale R. McCullough
    • 1
  1. 1.Department of Environmental Science, Policy and ManagementUniversity of California BerkeleyBerkeleyUSA
  2. 2.Center for Reproduction of Endangered SpeciesZoological Society of San DiegoSan DiegoUSA
  3. 3.Ornithology and MammalogyCalifornia Academy of SciencesSan FranciscoUSA