Phylogeography and Origin of Sheep Breeds in Northern China
Received: 25 April 2005 Accepted: 08 June 2005 DOI:
10.1007/s10592-005-8670-z Cite this article as: Ma, YH., Rao, SQ., Lu, SJ. et al. Conserv Genet (2006) 7: 117. doi:10.1007/s10592-005-8670-z Abstract
With the establishment of modern sheep production systems in China, various forms of hybridization with Western breeds and between native breeds have been utilized for genetic improvement. At the same time, the progressive destruction or deterioration of sheep habitat has accompanied urbanization in China. Together these factors have accelerated the loss of genetic diversity, or even resulted in the extinction of some indigenous breeds. It is therefore important that efficient strategies for surveillance, evaluation, conservation and utilization of available genetic resources are developed for this species. In this study, a total of 30 microsatellite markers were used to assess genetic diversity for 12 native breeds and one Western sheep breed in Northern China. The high polymorphism information contents at the 30 markers, varying from averages of 0.519 to 0.666 for the 13 breeds, imply the retention of natural variation from source populations in the domestic breeds from different geographic regions in China. Analysis of genetic differentiation revealed substantial divergence among these breeds. Neutrality tests indicated that more than one third of the 30 loci were in departure from neutrality, implying that some evolutionary forces (e.g. selection and migration) had acted on these populations. Phylogenetic and phylogeographic analyses displayed a remarkable degree of consistency between geographic origins, breeding histories and the pattern of genetic differentiation.
Keywords genetic distance genetic diversity microsatellite phylogeography sheep References Arranz, JJ, Bayon, Y, San Primitivo, F 1998 Genetic relationships among Spanish sheep using microsatellites Anim. Genet. 29 435 440 PubMed Google Scholar
Barker JSF (1994) A global protocol for determining genetic
distance among domestic livestock breeds.
Proceedings of the 5th world congress on genetics applied to livestock production, Guelph, Ontario, Canada, 21, 501–508.
Bonnet E, Van de Peer Y (2002) zt: a software tool for simple and partial Mentel tests.
Journal of Statistical Software
, 1–12. Available at
Botstein, D, White, RL, Skolnick, M, Davis, RW 1980 Construction of a genetic linkage map in man using restriction fragment length polymorphisms Am. J. Hum. Genet. 32 314 331 PubMed Google Scholar Chang, H, Geng, SM, Wu, B, Li, XY, Chen, YC 1989 Efficiencies of different sampling strategies for estimation of gene frequencies in Chinese cattle breeds Acta Universitatis Septentrionali Occidentali Agriculture 17 30 37 Google Scholar Chen, YC 1995 The significance of conservation of domestic animal diversity in China Biodiversity (China) 3 143 146 Google Scholar Chen, YC, Cao, HH 2001 Diversity of Chinese yellow cattle breeds and their conservation Biodiversity (China) 9 275 283 Google Scholar Crandall, KA, Bininda-Emonds, OR, Mace, GM, Wayne, RK 2000 Considering evolutionary processes in conservation biology Trends Ecol. Evol. 15 290 295 CrossRef PubMed Google Scholar Crawford, AM, Dodds, KG, Ede, AJ, Pierson, CA, Montgomery, GW, Garmonsway, HG, Beattie, AE, Davies, K, Maddox, JF, Kappes, SW, Stone, RT, Nguyen, TC, Penty, JM, Lord, EA, Broom, JE, Buitkamp, J, Schwaiger, W, Epplen, JT, Matthew, P, Matthews, ME, Hulme, DJ, Beh, KJ, McGraw, RA, Beattie, CW 1995 An autosomal genetic linkage map of the sheep genome Genetics 140 703 724 PubMed Google Scholar Crawford, AM, Montgomery, GW, Pierson, CA, Brown, T, Dodds, KG, Sunden, S, Henry, HM, Ede, AJ, Swarbrick, PA, Berryman, T, Penty, JM, Hill, DF 1994 Sheep linkage mapping: Nineteen linkage groups derived from the analysis of paternal half-sib families Genetics 137 573 579 PubMed Google Scholar Gortari, MJ, Freking, BA, Kappes, SM, Leymaster, KA, Crawford, AM, Stone, RT, Beattie, CW 1997 Extensive genomic conservation of cattle microsatellite heterozygosity in sheep Anim. Genet. 28 274 290 CrossRef PubMed Google Scholar Fan, B, Wang, ZG, Li, YJ, Zhao, XL, Liu, B, Zhao, SH, Yu, M, Li, MH, Chen, SL, Xiong, TA, Li, K 2002 Genetic variation analysis within and among Chinese indigenous swine populations using microsatellite markers Anim. Genet. 33 422 427 CrossRef PubMed Google Scholar Feng, WQ, Ma, YH, Chen, YC 1997 Views on genetic resources of domestic animals in China Chin. J. Anim. Vet. Sci. 28 300 303 Google Scholar Fu, YX, Li, WH 1993 Statistical tests of neutrality of mutations Genetics 133 693 709 PubMed Google Scholar Fulton, JE, Delany, ME 2003 Genetics. Poultry genetic resources – operation rescue needed Science 300 1667 1668 CrossRef PubMed Google Scholar Gong, YF, Li, XL, Liu, ZZ, Li, JQ 2002 Studies of random amplifiedpolymorphic DNA (RAPD) of main indigenous sheep breeds in China Hereditas (Beijing) 24 423 426 Google Scholar Guti’errez-Espeleta, GA, Kalinowski, ST, Boyce, WM, Hedrick, PW 2000 Genetic variation and population structure in desert bighorn sheep: Implications for conservation Conserv. Genet. 1 3 15 Google Scholar Hartl, DL, Clark, AG 1989Principles of Population Genetics Sinauer Associates, Inc. Sunderland, Massachusetts Google Scholar Li, K, Chen, Y, Moran, C, Fan, B, Zhao, S, Peng, Z 2000 Analysis of diversity and genetic relationships between four Chinese indigenous pig breeds and one Australian commercial pig breed Anim. Genet. 31 322 325 PubMed Google Scholar Li, MH, Zhao, SH, Bian, C, Wang, HS, Wei, H, Liu, B, Yu, M, Fan, B, Chen, SL, Zhu, MJ, Li, SJ, Xiong, TA, Li, K 2002 Genetic relationships among twelve Chinese indigenous goat populations based on microsatellite analysis Genet. Sel. Evol. 34 729 744 CrossRef PubMed Google Scholar Long, JR, Qiu, XP, Zeng, FT, Tang, LM, Zhang, YP 2003 Origin of rabbit (Oryctolagus cuniculus) in China: Evidence from mitochondrial DNA control region sequence analysis Anim. Genet. 34 82 87 CrossRef PubMed Google Scholar Ma, YH, Cao, HH, Chen, YC, Wang, D, Lu, SJ 2003a Analysis of genetic diversity in several cattle breeds Agric. Sci. China 2 804 808 Google Scholar Ma, YH, Xu, GF, Wang, DY, Liu, HL 2003b Dynamic changes in animal genetic resources in China Agric. Sci. China 2 80 84 Google Scholar Mburu, DN, Ochieng, JW, Kuria, SG, Jianlin, H, Kaufmann, B, Rege, JE, Hanotte, O 2003 Genetic diversity and relationships of indigenous Kenyan camel (Camelus dromedarius) populations: Implications for their classification Anim. Genet. 34 26 32 CrossRef PubMed Google Scholar Nei, M 1972 Genetic distance between population Am. Nat. 106 283 293 CrossRef Google Scholar Nei, M, Tajima, F, Tateno, Y 1983 Accuracy of estimated phylogenetic trees from molecular data. II. Gene frequency data J. Mol. Evol. 19 153 170 CrossRef PubMed Google Scholar
Ota T (1993)
Dispan: Genetic distance and phylogenetic analysis
Pennsylvania State University, University Park, PA. Available at:
Pang, YZ, Zhou, YY, Xu, TS, Zhang, HW, Wang, DY 1998 Karyotype analysis of Henan Small-Tail Han sheep Zhong Guo Xu Mu Zha Zhi (Chin. J. Anim. Sci.) 34 29 30 Google Scholar Rao, S, Notter, DR 2000 Genetic analysis of litter size in Targhee, Suffolk, and Polypay sheep J. Anim. Sci. 78 2113 2120 PubMed Google Scholar Sambrook, J, Fritsch, EF, Maniatis, T 1989Molecular Cloning: A Laboratory Manual2 Cold Spring Harbor Laboratory Press New York Google Scholar
Schneider S, Roessli D, Excoffier L (2000)
Arlequin: A software for population genetics data analysis. Ver 2.000
Genetics and Biometry Lab, Department of Anthropology, University of Geneva. Available at:
Simon, DL 1999 Better decisions in conservation of farm animal genetic resources by use of international sources of information Information in Decision Making in Agricultural Research and Practice/IAALD QB XLIV 1/2 59 65 Google Scholar Sneath, PHA, Sokal, RR 1973Numerical Taxonomy Freeman San Francisco Google Scholar Sun, W, Chang, H, Ren, ZJ, Yang, ZP, Geng, RQ, Tsunoda, K 2003a Studies on discriminants phylogenetic extraction of sheep populations in East and South of Central Asia Acta Veterinaria et Zootechnica Sinica 34 132 138 Google Scholar Sun, W, Chang, H, Yang, ZP, Geng, RQ, Lu, SX, Chang, GB, Xu, W, Wang, HY, Ni, DX, Fan, BS, Tsunoda, K 2003b Phylogenetic relationships between sheep populations in East and South of Central Asia Scientia Agriculture Sinica 36 94 98 Google Scholar Tsunoda, K, Nozawa, K, Madeda, Y, Tanabe, Y 1999 External morphological characters and blood protein and non-protein polymorphism of Native sheep in Central Mongolia Rep. Soc. Res. Native Livestock 17 63 82 Google Scholar
Weir BS (1996)
Genetic Data Analysis 2: Methods for Discrete Population Genetic Data
. Sinauer Associates, Sunderland, Massachusetts. Software GDA available at:
Yeh FC, Boyle TJB (1997) Population genetic analysis of co-dominant and dominant markers and quantitative traits.
Belgian Journal of Botany
, 157. The corresponding software POPGENE available at:
Zheng, PL 1988Sheep and Goat Breeds in China Shanghai Scientific & Technology Publishers Shanghai Google Scholar