Article

Clinical & Experimental Metastasis

, Volume 22, Issue 1, pp 47-59

Syngeneic mouse mammary carcinoma cell lines: Two closely related cell lines with divergent metastatic behavior

  • Alexander D. BorowskyAffiliated withDepartment of Medical Pathology and Laboratory Medicine, UC Davis School of MedicineCenter for Comparative Medicine, UC Davis
  • , Ruria NambaAffiliated withDepartment of Medical Pathology and Laboratory Medicine, UC Davis School of Medicine
  • , Lawrence J.T. YoungAffiliated withDepartment of Medical Pathology and Laboratory Medicine, UC Davis School of MedicineCenter for Comparative Medicine, UC Davis
  • , Kent W. HunterAffiliated withLaboratory of Population Genetics, Division of Cancer Epidemiology and Genetics, National Cancer Institute
  • , J. Graeme HodgsonAffiliated withDepartment of Neurological Surgery, UC San Francisco
  • , Clifford G. TepperAffiliated withDepartment of Medical Pathology and Laboratory Medicine, UC Davis School of MedicineDepartment of Biochemistry and Molecular Medicine, UC Davis School of Medicine
  • , Erik T. McGoldrickAffiliated withDepartment of Medical Pathology and Laboratory Medicine, UC Davis School of MedicineCenter for Comparative Medicine, UC Davis
  • , William J. MullerAffiliated withMolecular Oncology Group, McGill University, Royal Victoria Hospital
  • , Robert D. CardiffAffiliated withDepartment of Medical Pathology and Laboratory Medicine, UC Davis School of MedicineCenter for Comparative Medicine, UC Davis
    • , Jeffrey P. GreggAffiliated withDepartment of Medical Pathology and Laboratory Medicine, UC Davis School of Medicine

Rent the article at a discount

Rent now

* Final gross prices may vary according to local VAT.

Get Access

Abstract

Two cell lines, Met-1fvb2 and DB-7fvb2, with different metastatic potential, were derived from mammary carcinomas in FVB/N-Tg(MMTV-PyVmT) and FVB/N-Tg(MMTV-PyVmT Y315F/Y322F ) mice, transplanted into syngeneic FVB/N hosts and characterized. The lines maintain a stable morphological and biological phenotype after multiple rounds of in vitro culture and in vivo transplantation. The Met-1fvb2 line derived from a FVB/N-Tg(MMTV-PyVmT) tumor exhibits invasive growth and 100% metastases when transplanted into the females FVB/N mammary fat pad. The DB-7fvb2 line derived from the FVB/N-Tg(MMTV-PyVmT Y315F/Y322F ) with a “double base” modification at Y315F/Y322F exhibits more rapid growth when transplanted into the mammary fat pad, but a lower rate of metastasis (17%). The Met1fvb2 cells show high activation of AKT, while DB-7fvb2 cells show very low levels of AKT activation. The DNA content and gene expression levels of both cell lines are stable over multiple generations. Therefore, these two cell lines provide a stable, reproducible resource for the study of metastasis modulators, AKT molecular pathway interactions, and gene target and marker discovery.

Keywords

Akt breast breast carcinoma cell line metastasis comparative genomic hybridization ERBB2 gene expression analysis LY294002 mammary fat pad mouse mammary tumor virus long terminal repeat oncogene orthotopic phosphatidylinositol 3 kinase polyoma middle T pulmonary metastasis Sept9 Spp1 osteopontin Opn syngeneic transgenic tumor transplant