Chromosome Research

, Volume 21, Issue 8, pp 765–779

Emerin and histone deacetylase 3 (HDAC3) cooperatively regulate expression and nuclear positions of MyoD, Myf5, and Pax7 genes during myogenesis

Article

DOI: 10.1007/s10577-013-9381-9

Cite this article as:
Demmerle, J., Koch, A.J. & Holaska, J.M. Chromosome Res (2013) 21: 765. doi:10.1007/s10577-013-9381-9

Abstract

The spatial organization of chromatin is critical in establishing cell-type dependent gene expression programs. The inner nuclear membrane protein emerin has been implicated in regulating global chromatin architecture. We show emerin associates with genomic loci of muscle differentiation promoting factors in murine myogenic progenitors, including Myf5 and MyoD. Prior to their transcriptional activation Myf5 and MyoD loci localized to the nuclear lamina in proliferating progenitors and moved to the nucleoplasm upon transcriptional activation during differentiation. The Pax7 locus, which is transcribed in proliferating progenitors, localized to the nucleoplasm and Pax7 moved to the nuclear lamina upon repression during differentiation. Localization of Myf5, MyoD, and Pax7 to the nuclear lamina and proper temporal expression of these genes required emerin and HDAC3. Interestingly, activation of HDAC3 catalytic activity rescued both Myf5 localization to the nuclear lamina and its expression. Collectively, these data support a model whereby emerin facilitates repressive chromatin formation at the nuclear lamina by activating the catalytic activity of HDAC3 to regulate the coordinated spatiotemporal expression of myogenic differentiation genes.

Keywords

Emerin HDAC3 Chromatin remodeling Myogenesis Nuclear organization nuclear Lamina Nucleoskeleton 

Abbreviations

ChIP

Chromatin immunoprecipitation

ESCs

Embryonic stem cells

FISH

Fluorescent in situ hybridization

HDAC3

Histone deacetylase 3

INM

Inner nuclear membrane

LAD

Lamina-associated domain

LASs

Lamina-associated sequences

LBR

Lamin B receptor

Myf5

Myogenic factor 5

MyoD

Myoblast determination protein 1

NCoR

Nuclear corepressor complex

NPC

Nuclear pore complex

ONM

Outer nuclear membrane

Pax3

Paired box 3

Pax7

Paired box 7

PRC2

Polycomb repressive complex 2

X-EDMD

X-linked Emery–Dreifuss muscular dystrophy

Supplementary material

10577_2013_9381_MOESM1_ESM.doc (77 kb)
ESM 1(DOC 77 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  1. 1.Department of Medicine, Section of CardiologyThe University of ChicagoChicagoUSA
  2. 2.Committee on Genetics, Genomics and Systems BiologyThe University of ChicagoChicagoUSA
  3. 3.Committee on Development, Regeneration and Stem Cell BiologyThe University of ChicagoChicagoUSA
  4. 4.Department of BiochemistryUniversity of OxfordOxfordUK

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