Chromosome Research

, Volume 18, Issue 1, pp 19-34

Molecular analysis of the replication program in unicellular model organisms

  • M. K. RaghuramanAffiliated withDepartment of Genome Sciences, University of Washington Email author 
  • , Bonita J. BrewerAffiliated withDepartment of Genome Sciences, University of Washington


Eukaryotes have long been reported to show temporal programs of replication, different portions of the genome being replicated at different times in S phase, with the added possibility of developmentally regulated changes in this pattern depending on species and cell type. Unicellular model organisms, primarily the budding yeast Saccharomyces cerevisiae, have been central to our current understanding of the mechanisms underlying the regulation of replication origins and the temporal program of replication in particular. But what exactly is a temporal program of replication, and how might it arise? In this article, we explore this question, drawing again on the wealth of experimental information in unicellular model organisms.


origin replication timing temporal program yeast