Chromosome Research

, 17:927

Extensive conservation of genomic imbalances in canine transmissible venereal tumors (CTVT) detected by microarray-based CGH analysis

  • Rachael Thomas
  • Clare Rebbeck
  • Armand M. Leroi
  • Austin Burt
  • Matthew Breen
Article

DOI: 10.1007/s10577-009-9080-8

Cite this article as:
Thomas, R., Rebbeck, C., Leroi, A.M. et al. Chromosome Res (2009) 17: 927. doi:10.1007/s10577-009-9080-8

Abstract

Canine transmissible venereal tumor (CTVT) is an intriguing cancer that is transmitted naturally as an allograft by transplantation of viable tumor cells from affected to susceptible dogs. At least initially, the tumor is able to evade the host's immune response; thus, CTVT has potential to provide novel insights into tumor immunobiology. The nature of CTVT as a “contagious” cancer, originating from a common ancestral source of infection, has been demonstrated previously by a series of studies comparing geographically distinct tumors at the molecular level. While these studies have revealed that apparently unrelated tumors share a striking degree of karyotypic conservation, technological restraints have limited the ability to investigate the chromosome composition of CTVTs in any detail. We present characterization of a strategically selected panel of CTVT cases using microarray-based comparative genomic hybridization analysis at ~one-megabase resolution. These data show for the first time that the tumor presents with an extensive range of non-random chromosome copy number aberrations that are distributed widely throughout the dog genome. The majority of abnormalities detected were imbalances of small subchromosomal regions, often involving centromeric and telomeric sequences. All cases also showed the sex chromosome complement XO. There was remarkable conservation in the cytogenetic profiles of the tumors analyzed, with only minor variation observed between different cases. These data suggest that the CTVT genome demonstrates a vast degree of both structural and numerical reorganization that is maintained during transmission among the domestic dog population.

Keywords

comparative genomic hybridization (CGH) canine transmissible venereal tumor chromosome microarray 

Abbreviations

aCGH

array-based comparative genomic hybridization

BAC

bacterial artificial chromosome

CFA

Canis familiaris

CGH

comparative genomic hybridization

CHORI

Children's Hospital Oakland Research Institute

CNA

copy number aberration

CTVT

canine transmissible venereal tumor

DAPI

4′,6-diamidino-2-phenylindole

DFTD

devil facial tumor disease

FISH

fluorescence in situ hybridization

LINE-1

long interspersed nuclear element

Mb

megabase

RPCI

Roswell Park Cancer Institute

Supplementary material

10577_2009_9080_MOESM1_ESM.xls (410 kb)
Supplementary Table1Thomas et al cTVT aCGH SOM (XLS 409 kb)

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Rachael Thomas
    • 1
    • 2
  • Clare Rebbeck
    • 3
    • 4
  • Armand M. Leroi
    • 3
  • Austin Burt
    • 3
  • Matthew Breen
    • 1
    • 2
    • 5
  1. 1.Department of Molecular Biomedical Sciences, College of Veterinary MedicineNorth Carolina State UniversityRaleighUSA
  2. 2.Center for Comparative Medicine and Translational ResearchNorth Carolina State UniversityRaleighUSA
  3. 3.Department of BiologyImperial College LondonAscotUK
  4. 4.Cold Spring Harbor LaboratoryCold Spring HarborUSA
  5. 5.Cancer Genetics ProgramUNC Lineberger Comprehensive Cancer CenterChapel HillUSA

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