Chromosome Research

, Volume 16, Issue 8, pp 1203–1214

Histone modifications associated with both A and B chromosomes of maize

  • Weiwei Jin
  • Jonathan C. Lamb
  • Wenli Zhang
  • Bozena Kolano
  • James A. Birchler
  • Jiming Jiang
Article

DOI: 10.1007/s10577-008-1269-8

Cite this article as:
Jin, W., Lamb, J.C., Zhang, W. et al. Chromosome Res (2008) 16: 1203. doi:10.1007/s10577-008-1269-8

Abstract

We report the distribution of several histone modifications along the arms and in centromeric regions of somatic chromosomes of maize, including the supernumerary B chromosome. Acetylated H3 and H4 as well as H3K4me2, modifications associated with euchromatin, were enriched in the distal parts of the A chromosomes, but were progressively depleted toward the centromeres of the A chromosomes and were depleted in the heterochromatic portions of the B chromosome. Classical histone modifications associated with heterochromatin, including H3K9me2, H3K27me1 and H3K27me2, were distributed throughout both A and B chromosomes. However, H3K27me2 showed a reduced level on the B chromosome compared with the A chromosomes and was not associated with some classes of constitutive heterochromatin. We monitored the presence of each histone modification in the centromeric regions using a YFP-tagged centromere-specific histone, CENH3. We observed the presence of H3K9me2 and absence of H3K4me2 in the centromeric regions of both A and B chromosomes of maize, which is in contrast to the presence of H3K4me2 and absence of H3K9me2 in animal centromeres. These results show a diversity of epigenetic modifications associated with centromeric chromatin in different eukaryotes.

Key words

acetylation B chromosome centromere histone methylation maize Zea maysi 

Abbreviations

CENH3

centromere-specific histone H3

DAPI

4′,6-diamidino-2-phenylindole

FISH

fluorescence in-situ hybridization

YFP

yellow fluorescent protein

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Weiwei Jin
    • 1
    • 2
  • Jonathan C. Lamb
    • 3
  • Wenli Zhang
    • 1
  • Bozena Kolano
    • 1
  • James A. Birchler
    • 3
  • Jiming Jiang
    • 1
  1. 1.Department of HorticultureUniversity of Wisconsin-MadisonMadisonUSA
  2. 2.National Maize Improvement Center of China, Key Laboratory of Crop Genetic Improvement and Genome of Ministry of AgricultureChina Agricultural UniversityBeijingP. R. China
  3. 3.Division of Biological ScienceUniversity of MissouriColumbiaUSA

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