Chromosome Research

, Volume 16, Issue 4, pp 649–673

The nuclear organization of Polycomb/Trithorax group response elements in larval tissues of Drosophila melanogaster

  • Elena Fedorova
  • Nicolas Sadoni
  • Ina K. Dahlsveen
  • Jeannette Koch
  • Elisabeth Kremmer
  • Dirk Eick
  • Renato Paro
  • Daniele Zink
Article

DOI: 10.1007/s10577-008-1218-6

Cite this article as:
Fedorova, E., Sadoni, N., Dahlsveen, I.K. et al. Chromosome Res (2008) 16: 649. doi:10.1007/s10577-008-1218-6

Abstract

We analysed the nuclear organization of the Polycomb/Trithorax group response element (PRE/TRE) Fab-7 and of other PRE/TREs in larval tissues of D. melanogaster. The results show that pairing/clustering of transgenic and endogenous Fab-7 elements and of other endogenous PRE/TREs occurs only to a limited degree in a highly locus-specific and tissue-specific manner. However, transgenic Fab-7 elements as well as the Fab-7-regulated Abd-B gene and other endogenous loci preferentially occupied defined nuclear regions. Preferred association with the nuclear periphery was observed in the inactive state. However, also in the active state, Fab-7 was often found associated with the nuclear periphery as well as with the boundary of heterochromatin in a fly line- and tissue-specific manner. The boundary between heterochromatin and euchromatin revealed a highly complex architecture in the three-dimensional nuclear space with a close juxtaposition of active and repressed domains. The results suggest that such complex architectures create nuclear microenvironments sustaining specific states of activity of defined PRE/TREs. However, the data also show that the positional behaviour of the transgenic Fab-7 element does not apply to PRE/TREs in general. Altogether, this finding and the highly locus-, tissue-, and fly line-specific behaviour with regard to nuclear positioning and pairing/clustering suggest that the relationships between nuclear organization and functional regulation of PRE/TREs are highly complex and that simple models making general predictions might not be appropriate.

Key words

Fab-7kissingnuclear organizationPolycomb groupPRE/TRE

Supplementary material

Copyright information

© Springer 2008

Authors and Affiliations

  • Elena Fedorova
    • 1
    • 2
  • Nicolas Sadoni
    • 1
  • Ina K. Dahlsveen
    • 3
    • 4
  • Jeannette Koch
    • 1
  • Elisabeth Kremmer
    • 5
  • Dirk Eick
    • 6
  • Renato Paro
    • 7
  • Daniele Zink
    • 1
    • 8
  1. 1.Department Biologie IILudwig-Maximilians-Universität MünchenPlanegg-MartinsriedGermany
  2. 2.Department of Sensory PhysiologyRussian Academy of Sciences, I.P. Pavlov Institute of PhysiologySt. PetersburgRussia
  3. 3.Adolf-Butenandt-InstitutLudwig-Maximilians-Universität MünchenMünchenGermany
  4. 4.VedbaekDenmark
  5. 5.GSF-Forschungszentrum für Umwelt und GesundheitInstitut für Molekulare ImmunologieMünchenGermany
  6. 6.GSF-Forschungszentrum für Umwelt und GesundheitInstitut für Klinische Molekularbiologie und TumorgenetikMünchenGermany
  7. 7.Department of Biosystems Science and EngineeringETH ZurichBaselSwitzerland
  8. 8.Institute of Bioengineering and NanotechnologySingapore