Chromosome Research

, Volume 13, Issue 2, pp 113–122

The proto-oncogene C-KIT maps to canid B-chromosomes

  • Alexander S. Graphodatsky
  • Anna V. Kukekova
  • Dmitry V. Yudkin
  • Vladimir A. Trifonov
  • Nadezhda V. Vorobieva
  • Violetta R. Beklemisheva
  • Polina L. Perelman
  • Daria A. Graphodatskaya
  • Lyudmila N. Trut
  • Fengtang Yang
  • Malcolm A. Ferguson-Smith
  • Gregory M. Acland
  • Gustavo D. Aguirre
Article

DOI: 10.1007/s10577-005-7474-9

Cite this article as:
Graphodatsky, A.S., Kukekova, A.V., Yudkin, D.V. et al. Chromosome Res (2005) 13: 113. doi:10.1007/s10577-005-7474-9

Abstract

Plant and animal karyotypes sometimes contain variable elements, that are referred to as additional or B-chromosomes. It is generally believed that B-chromosomes lack major genes and represent parasitic and selfish elements of a genome. Here we report, for the first time, the localization of a gene to B-chromosomes of mammals: red fox (Vulpes vulpes) and two subspecies of raccoon dog (Nyctereutes procyonoides). Identification of the proto-oncogene C-KIT on B-chromosomes of two Canidae species that diverged from a common ancestor more than 12.5 million years ago argues against the current view of B-chromosomes. Analyses of fox B-chromosomal C-KIT gene from a flow-sorted fox B-chromosome-specific library revealed the presence of intron–exon boundaries and high identity between sequenced regions of canine and fox B-chromosomal C-KIT copies. Identification of C-KIT gene on all B-chromosomes of two canid species provides new insight into the origin and evolution of supernumeraries and their potential role in the genome.

Key words

B-chromosomeCanidaeC-KIToncogene

Copyright information

© Springer Science + Business Media, Inc. 2005

Authors and Affiliations

  • Alexander S. Graphodatsky
    • 1
  • Anna V. Kukekova
    • 2
  • Dmitry V. Yudkin
    • 1
  • Vladimir A. Trifonov
    • 1
  • Nadezhda V. Vorobieva
    • 1
  • Violetta R. Beklemisheva
    • 1
  • Polina L. Perelman
    • 1
  • Daria A. Graphodatskaya
    • 1
  • Lyudmila N. Trut
    • 1
  • Fengtang Yang
    • 3
  • Malcolm A. Ferguson-Smith
    • 3
  • Gregory M. Acland
    • 2
  • Gustavo D. Aguirre
    • 4
  1. 1.Institute of Cytology and GeneticsNovosibirskRussia
  2. 2.James A. Baker Institute for Animal HealthCollege of Veterinary Medicine, Cornell UniversityIthacaUSA
  3. 3.Centre for Veterinary Science, Department of Clinical Veterinary MedicineUniversity of CambridgeUnited Kingdom
  4. 4.School of Veterinary MedicineUniversity of PennsylvaniaUSA