Chromosome Research

, Volume 13, Issue 2, pp 113–122

The proto-oncogene C-KIT maps to canid B-chromosomes

Authors

    • Institute of Cytology and Genetics
  • Anna V. Kukekova
    • James A. Baker Institute for Animal HealthCollege of Veterinary Medicine, Cornell University
  • Dmitry V. Yudkin
    • Institute of Cytology and Genetics
  • Vladimir A. Trifonov
    • Institute of Cytology and Genetics
  • Nadezhda V. Vorobieva
    • Institute of Cytology and Genetics
  • Violetta R. Beklemisheva
    • Institute of Cytology and Genetics
  • Polina L. Perelman
    • Institute of Cytology and Genetics
  • Daria A. Graphodatskaya
    • Institute of Cytology and Genetics
  • Lyudmila N. Trut
    • Institute of Cytology and Genetics
  • Fengtang Yang
    • Centre for Veterinary Science, Department of Clinical Veterinary MedicineUniversity of Cambridge
  • Malcolm A. Ferguson-Smith
    • Centre for Veterinary Science, Department of Clinical Veterinary MedicineUniversity of Cambridge
  • Gregory M. Acland
    • James A. Baker Institute for Animal HealthCollege of Veterinary Medicine, Cornell University
  • Gustavo D. Aguirre
    • School of Veterinary MedicineUniversity of Pennsylvania
Article

DOI: 10.1007/s10577-005-7474-9

Cite this article as:
Graphodatsky, A.S., Kukekova, A.V., Yudkin, D.V. et al. Chromosome Res (2005) 13: 113. doi:10.1007/s10577-005-7474-9

Abstract

Plant and animal karyotypes sometimes contain variable elements, that are referred to as additional or B-chromosomes. It is generally believed that B-chromosomes lack major genes and represent parasitic and selfish elements of a genome. Here we report, for the first time, the localization of a gene to B-chromosomes of mammals: red fox (Vulpes vulpes) and two subspecies of raccoon dog (Nyctereutes procyonoides). Identification of the proto-oncogene C-KIT on B-chromosomes of two Canidae species that diverged from a common ancestor more than 12.5 million years ago argues against the current view of B-chromosomes. Analyses of fox B-chromosomal C-KIT gene from a flow-sorted fox B-chromosome-specific library revealed the presence of intron–exon boundaries and high identity between sequenced regions of canine and fox B-chromosomal C-KIT copies. Identification of C-KIT gene on all B-chromosomes of two canid species provides new insight into the origin and evolution of supernumeraries and their potential role in the genome.

Key words

B-chromosomeCanidaeC-KIToncogene
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Copyright information

© Springer Science + Business Media, Inc. 2005