Cellular and Molecular Neurobiology

, Volume 34, Issue 4, pp 479–489

Cells Isolated from Human Glioblastoma Multiforme Express Progesterone-Induced Blocking Factor (PIBF)

  • Dobroslav Kyurkchiev
  • Emanuil Naydenov
  • Kalina Tumangelova-Yuzeir
  • Ekaterina Ivanova-Todorova
  • Kalina Belemezova
  • Ivan Bochev
  • Krasimir Minkin
  • Milena Mourdjeva
  • Tsvetelina Velikova
  • Sevdalin Nachev
  • Stanimir Kyurkchiev
Original Research

DOI: 10.1007/s10571-014-0031-3

Cite this article as:
Kyurkchiev, D., Naydenov, E., Tumangelova-Yuzeir, K. et al. Cell Mol Neurobiol (2014) 34: 479. doi:10.1007/s10571-014-0031-3

Abstract

Glioblastoma multiforme (GBM) is the most common and malignant tumor in the central nervous system. One of the contemporary hypotheses postulates that its pathogenesis is associated with the cancer stem cells (CSCs) which originate from mutations in the normal neural stem cells residing in their specific “niches.” Simultaneously with its aggressive development the tumor suppresses the local immune system by different secreted and/or cell expressed factors. Progesterone-induced blocking factor (PIBF) is an immunomodulatory protein with known role in the regulation of the immune response in the reproductive system. Expression of PIBF has been described in some tumors as one of the factors suppressing the anti-tumor immunity. The aim of the present study was to check for the expression of PIBF from cells isolated from six GBMs. To characterize the cultured cells and to study the PIBF expression confocal microscopy, flow cytometry, ELISA, and real-time PCR were used. The results obtained showed expression of markers typical for cancer CSCs and secretion of interleukin 6 by the GBM-derived cultured cells. The results convincingly prove that PIBF is intracellularly expressed by the cultured cells from the all six GBM samples, and this fact is confirmed by three different methods—flow cytometry, confocal microscopy, and real-time PCR. This paper reports for the first time the expression of PIBF by GBM-derived cells cultured in vitro and reveals a new aspect of the immunosuppressive mechanism used by GBM in escaping the immune control.

Keywords

Glioblastoma multiforme Cancer stem cells Immunosuppression Progesterone-induced blocking factor 

Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Dobroslav Kyurkchiev
    • 1
  • Emanuil Naydenov
    • 2
  • Kalina Tumangelova-Yuzeir
    • 1
  • Ekaterina Ivanova-Todorova
    • 1
  • Kalina Belemezova
    • 3
  • Ivan Bochev
    • 4
  • Krasimir Minkin
    • 2
  • Milena Mourdjeva
    • 5
  • Tsvetelina Velikova
    • 1
  • Sevdalin Nachev
    • 6
  • Stanimir Kyurkchiev
    • 3
    • 4
  1. 1.Department of Clinical Laboratory and Clinical Immunology, University Hospital “St. Ivan Rilski”Medical University SofiaSofiaBulgaria
  2. 2.Clinic of Neurosurgery, University Hospital “St. Ivan Rilski”Medical University SofiaSofiaBulgaria
  3. 3.Tissue Bank “BulGen”SofiaBulgaria
  4. 4.Ob/Gyn Hospital “Dr. Shterev”SofiaBulgaria
  5. 5.Department of Molecular Immunology, Institute of Biology and Immunology of ReproductionBulgarian Academy of SciencesSofiaBulgaria
  6. 6.Laboratory of Clinical Pathology, University Hospital “St. Ivan Rilski”Medical University SofiaSofiaBulgaria

Personalised recommendations