Original Research

Cellular and Molecular Neurobiology

, Volume 32, Issue 3, pp 467-475

First online:

Protective Effects of Adeno-associated Virus Mediated Brain-derived Neurotrophic Factor Expression on Retinal Ganglion Cells in Diabetic Rats

  • Yan GongAffiliated withDepartment of Ophthalmology, Chinese PLA General Hospital
  • , Zhan-Ping ChangAffiliated withDepartment of Pathology, Aerospace 731 Hospital
  • , Ruo-Tong RenAffiliated withInstitute of Biophysics, Chinese Academy of Sciences
  • , Shi-hui WeiAffiliated withDepartment of Ophthalmology, Chinese PLA General Hospital
  • , Huan-Fen ZhouAffiliated withDepartment of Ophthalmology, The First Affiliated Hospital of General Hospital of PLA
  • , Xiao-fei ChenAffiliated withDepartment of Ophthalmology, Chinese PLA General Hospital
  • , Bao-ke HouAffiliated withDepartment of Ophthalmology, Chinese PLA General Hospital
  • , Xin JinAffiliated withDepartment of Ophthalmology, Chinese PLA General Hospital
  • , Mao-nian ZhangAffiliated withDepartment of Ophthalmology, Chinese PLA General Hospital Email author 

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Abstract

Adeno-associated virus vector plasmid carrying the expression cassette of brain-derived neurotrophic factor (BDNF), pAAV-BDNF, was constructed and packaged into recombinant adeno-associated virus (rAAV-BDNF). The rAAV-BDNF was intravitreally injected into streptzotocin (STZ)-induced diabetic Sprague–Dawley (SD) Rats. Data showed that over-expression of BDNF could increase alive retinal ganglion cell (RGC) number and improve its function in streptzotocin(STZ)-induced diabetic rats, which might be a new method to treat diabetic neuropathy and retinopathy.

Keywords

Streptzotocin(STZ) Diabetic retinopathy Gene therapy Adeno-associated virus (AAV) Brain-derived neurotrophic factor (BDNF)