Cellular and Molecular Neurobiology

, Volume 30, Issue 6, pp 857–862

Ginsenoside Rb1 and Rg3 Attenuate Glucocorticoid-Induced Neurotoxicity

  • Sung-Ok Kim
  • Jung-Man You
  • Su-Jin Yun
  • Min-Sook Son
  • Kyong Nyon Nam
  • Joung-Woo Hong
  • Sun Yeou Kim
  • Sang Yoon Choi
  • Eunjoo H. Lee
Original Research

DOI: 10.1007/s10571-010-9513-0

Cite this article as:
Kim, SO., You, JM., Yun, SJ. et al. Cell Mol Neurobiol (2010) 30: 857. doi:10.1007/s10571-010-9513-0
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Abstract

Glucocorticoid (GC) hormones, increased in response to stress, can cause neuronal loss. We tested the effect of GC hormone on cell viability of neural SHSY-5Y cells and protective effects of ginsenoside Rb1 and Rg3 on the action of GC. We treated SHSY-5Y cells with increasing concentrations of synthetic GC dexamethasone (DEX; 10, 25, 50, and 100 nM) for 24 and 48 h, and then determined cell viability by using MTT assay. We then treated SHSY-5Y cells with DEX (100 nM) with or without the ginsenosides to examine their preventive effects on the cytotoxicity. To explore the underlying molecular mechanisms, we measured mRNA expression of bax and bcl-2 by using reverse transcriptase real-time PCR. SHSY-5Y cells treated with DEX significantly reduced cell viability as compared with control cells. In the presence of Rb1 or Rg3, DEX-induced cytotoxicity was effectively blocked. DEX considerably increased pro-apoptotic bax mRNA expression as compared with control cells. However, Rb1 and Rg3 completely blocked DEX-mediated up-regulation of bax expression. DEX significantly increased neuronal death in organotypic hippocampal slice cultures of rat brain with enhanced generation of ROS, which was effectively inhibited by ginsenoside Rb1 and Rg3. This suggests a potential role of the ginsenosides to target GC action in the brain.

Keywords

DexamethasoneGinsenosideGlucocorticoidNeuroprotectionNeurotoxicityOrganotypic hippocampal slice cultureReactive oxygen speciesSHSY-5YStress

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Sung-Ok Kim
    • 1
    • 3
  • Jung-Man You
    • 1
  • Su-Jin Yun
    • 1
    • 4
  • Min-Sook Son
    • 1
  • Kyong Nyon Nam
    • 1
  • Joung-Woo Hong
    • 1
  • Sun Yeou Kim
    • 1
  • Sang Yoon Choi
    • 2
  • Eunjoo H. Lee
    • 1
  1. 1.Department of Medical Science, Graduate School of East–West Medical Science; East–West Integrated Medical Science Research CenterKyung Hee UniversityYongin-siKorea
  2. 2.Korea Food Research InstituteSungnamKorea
  3. 3.Department of SurgeryUniversity of Texas Medical BranchGalvestoneUSA
  4. 4.Department of ChemistryThe Pennsylvania State UniversityUniversity ParkUSA