Cellular and Molecular Neurobiology

, Volume 29, Issue 1, pp 115–121

Interaction Between Syntaxin 8 and HECTd3, a HECT Domain Ligase

  • Lisheng Zhang
  • Liang Kang
  • William Bond
  • Nian Zhang
Original Paper

DOI: 10.1007/s10571-008-9303-0

Cite this article as:
Zhang, L., Kang, L., Bond, W. et al. Cell Mol Neurobiol (2009) 29: 115. doi:10.1007/s10571-008-9303-0

Abstract

Ubiquitination of proteins and their degradation within the proteasome has emerged as the major proteolytic mechanism used by mammalian cells to regulate cytosolic and nuclear protein levels. Substrate ubiquitylation is mediated by ubiquitin (Ub) ligases, also called E3 Ub ligases. HECT-E3 Ub ligases are characterized by the presence of a C-terminal HECT domain that contains the active site for Ub transfer onto substrates. Among the many E3 Ub ligases, the family homologous to E6-Ap C-terminus (HECT) E3 Ub ligases, which includes the yeast protein Rsp5p and the mammalian homolog NEDD4, AIP4/Itch, and Smurf, has been shown to ubiquitylate membrane proteins and, in some instances, to induce their degradation. In this report, we have identified Syntaxin 8 as a binding protein to a novel HECT domain protein, HECT domain containing 3 (HECTd3), by yeast two-hybrid screen. Besides HECT domain, HECTd3 contains an anaphase-promoting complex, subunit 10 (APC10) domain. Our co-immunoprecipitation experiments show that Syntaxin 8 directly interacts with HECTd3 and that the overexpression of HECTd3 promotes the ubiquitination of Syntaxin 8. Immunofluorescence results show that Syntaxin 8 and HECTd3 have similar subcellular localization.

Keywords

Syntaxin 8UbiquitinationHECT E3 ubiquitin ligasesHECTd3

Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Lisheng Zhang
    • 1
  • Liang Kang
    • 1
  • William Bond
    • 1
    • 2
  • Nian Zhang
    • 1
    • 3
  1. 1.Laboratory of Mammalian Developmental GeneticsVan Andel Research InstituteGrand RapidsUSA
  2. 2.Department of Biology and BiochemistryBath UniversityBathUK
  3. 3.Stower Institute for Medical ResearchKansas CityUSA