Cellulose

, Volume 19, Issue 6, pp 2093–2104

Structural characterisation of thiol-modified hyaluronans

  • Eva Hrabárová
  • Jozef Rychlý
  • Vlasta Sasinková
  • Katarína Valachová
  • Ivica Janigová
  • Katarína Csomorová
  • Ivo Juránek
  • Ladislav Šoltés
Original Paper

DOI: 10.1007/s10570-012-9775-4

Cite this article as:
Hrabárová, E., Rychlý, J., Sasinková, V. et al. Cellulose (2012) 19: 2093. doi:10.1007/s10570-012-9775-4

Abstract

Fourier-transform infrared spectroscopy and non-isothermal methods—chemiluminometry, differential scanning calorimetry, and differential thermogravimetry—were used to characterize potential structural changes of thiol-modified hyaluronans. Degradative conditions tested via rotational viscometry were first initiated applying oxidative Weissberger’s system in a reaction system under aerobic conditions. Several low-molecular-weight thiol compounds—cysteamine, l-cysteine, and N-acetyl-l-cysteine—were subsequently tested for their potential antioxidative effects against hyaluronan degradation. It was shown that different final values of dynamic viscosity of hyaluronan solutions were dependent on the thiol structure and its initial concentration. An idea has been put forward that together with the reduction of the hyaluronan molecular weight, which is a consequence of fragmentation, the degradation products might contain associated or even cross-linked structures. In the case of N-acetyl-l-cysteine application, the carbonaceous residue evidenced by differential thermogravimetry was increased when compared to that of intact hyaluronan.

Keywords

HyaluronanRotational viscometryFourier-transform infrared spectroscopyNon-isothermal methods

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Eva Hrabárová
    • 1
  • Jozef Rychlý
    • 2
  • Vlasta Sasinková
    • 3
  • Katarína Valachová
    • 4
  • Ivica Janigová
    • 2
  • Katarína Csomorová
    • 2
  • Ivo Juránek
    • 4
  • Ladislav Šoltés
    • 4
  1. 1.Department of Carbohydrate Enzymology, Institute of ChemistrySlovak Academy of SciencesBratislavaSlovak Republic
  2. 2.Polymer InstituteSlovak Academy of SciencesBratislavaSlovak Republic
  3. 3.Department of Analytical Chemistry, Institute of ChemistrySlovak Academy of SciencesBratislavaSlovak Republic
  4. 4.Institute of Experimental Pharmacology and ToxicologySlovak Academy of SciencesBratislavaSlovak Republic