Cell Biology and Toxicology

, Volume 29, Issue 1, pp 39–58

Stress reaction of kidney epithelial cells to inorganic solid-core nanoparticles

  • Blanka Halamoda Kenzaoui
  • Catherine Chapuis Bernasconi
  • Lucienne Juillerat-Jeanneret
Original Research

DOI: 10.1007/s10565-012-9236-8

Cite this article as:
Halamoda Kenzaoui, B., Chapuis Bernasconi, C. & Juillerat-Jeanneret, L. Cell Biol Toxicol (2013) 29: 39. doi:10.1007/s10565-012-9236-8

Abstract

A route of accumulation and elimination of therapeutic engineered nanoparticles (NPs) may be the kidney. Therefore, the interactions of different solid-core inorganic NPs (titanium-, silica-, and iron oxide-based NPs) were studied in vitro with the MDCK and LLC-PK epithelial cells as representative cells of the renal epithelia. Following cell exposure to the NPs, observations include cytotoxicity for oleic acid-coated iron oxide NPs, the production of reactive oxygen species for titanium dioxide NPs, and cell depletion of thiols for uncoated iron oxide NPs, whereas for silica NPs an apparent rapid and short-lived increase of thiol levels in both cell lines was observed. Following cell exposure to metallic NPs, the expression of the tranferrin receptor/CD71 was decreased in both cells by iron oxide NPs, but only in MDCK cells by titanium dioxide NPs. The tight association, then subsequent release of NPs by MDCK and LLC-PK kidney epithelial cells, showed that following exposure to the NPs, only MDCK cells could release iron oxide NPs, whereas both cells released titanium dioxide NPs. No transfer of any solid-core NPs across the cell layers was observed.

Keywords

Cytotoxicity Epithelial kidney cells Nanoparticles Oxidative stress Transepithelial transfer Transferrin receptor/CD71 

Abbreviations

Carboxy-H2DCFDA

5/6-Carboxy-2,7-dichloro-dihydro-fluorescein

DAPI

4′,6′-Diamidino-2-phenylindole

DLS

Dynamic light scattering

HBSS

Hank’s buffer solution

3H-T

Tritiated thymidine

LY

Lucifer Yellow

MTT

3,4,5-Dimethylthiazol-yl-2,5-diphenyl tetrazolium bromide

NEM

N-Ethyl-maleimide

NPs

Nanoparticles

ROS

Reactive oxygen species

TBHP

tert-Butyl hydroperoxide

TEER

Transepithelial electrical resistance

TEM

Transmission electron microscopy

USPIO

Ultrasmall superparamagnetic iron oxide

Supplementary material

10565_2012_9236_MOESM1_ESM.doc (1.2 mb)
ESM 1(DOC 1210 kb)

Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Blanka Halamoda Kenzaoui
    • 1
    • 2
  • Catherine Chapuis Bernasconi
    • 1
    • 2
  • Lucienne Juillerat-Jeanneret
    • 1
    • 2
  1. 1.University Institute of PathologyCentre Hospitalier Universitaire Vaudois (CHUV)LausanneSwitzerland
  2. 2.University of Lausanne (UNIL)LausanneSwitzerland