Cell Biology and Toxicology

, Volume 27, Issue 5, pp 333–342

Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles in human cardiac microvascular endothelial cells

Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles
  • Jing Sun
  • Shaochuang Wang
  • Dong Zhao
  • Fei Han Hun
  • Lei Weng
  • Hui Liu
Article

DOI: 10.1007/s10565-011-9191-9

Cite this article as:
Sun, J., Wang, S., Zhao, D. et al. Cell Biol Toxicol (2011) 27: 333. doi:10.1007/s10565-011-9191-9

Abstract

Wide applications and extreme potential of metal oxide nanoparticles (NPs) increase occupational and public exposure and may yield extraordinary hazards for human health. Exposure to NPs has a risk for dysfunction of the vascular endothelial cells. The objective of this study was to assess the cytotoxicity of six metal oxide NPs to human cardiac microvascular endothelial cells (HCMECs) in vitro. Metal oxide NPs used in this study included zinc oxide (ZnO), iron(III) oxide (Fe2O3), iron(II,III) oxide (Fe3O4), magnesium oxide (MgO), aluminum oxide (Al2O3), and copper(II) oxide (CuO). The cell viability, membrane leakage of lactate dehydrogenase, intracellular reactive oxygen species, permeability of plasma membrane, and expression of inflammatory markers vascular cell adhesion molecule-1, intercellular adhesion molecule-1, macrophage cationic peptide-1, and interleukin-8 in HCMECs were assessed under controlled and exposed conditions (12–24 h and 0.001–100 μg/ml of exposure). The results indicated that Fe2O3, Fe3O4, and Al2O3 NPs did not have significant effects on cytotoxicity, permeability, and inflammation response in HCMECs at any of the concentrations tested. ZnO, CuO, and MgO NPs produced the cytotoxicity at the concentration-dependent and time-dependent manner, and elicited the permeability and inflammation response in HCMECs. These results demonstrated that cytotoxicity, permeability, and inflammation in vascular endothelial cells following exposure to metal oxide nanoparticles depended on particle composition, concentration, and exposure time.

Keywords

CytotoxicityPermeabilityInflammationMetal oxide nanoparticlesVascular endothelial cells

Abbreviations

NPs

Nanoparticles

Fe2O3

Iron(III) oxide

Y2O3

Yttrium oxide

CeO2

Cerium oxide

ZnO

Zinc oxide

Fe3O4

Iron(II,III) oxide

MgO

Magnesium oxide

Al2O3

Aluminum oxide

CuO

Copper(II) oxide

ECM

Endothelial cell medium

HCMECs

Human cardiac microvascular endothelial cells

LDH

Lactate dehydrogenase

ROS

Reactive oxygen species

VCAM-1

Vascular cell adhesion molecule-1

ICAM-1

Intercellular adhesion molecule 1

MCP-1

Macrophage cationic peptide-1

IL-8

Interleukin-8

Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • Jing Sun
    • 1
  • Shaochuang Wang
    • 2
  • Dong Zhao
    • 1
  • Fei Han Hun
    • 1
  • Lei Weng
    • 1
  • Hui Liu
    • 3
  1. 1.Shanghai First Maternity and Infant HospitalTongji UniversityShanghaiChina
  2. 2.Department of Hepatobiliary Pancreatic SurgeryThe Huai’an First People’s Hospital Affiliated to Nanjin Medical UniversityJiangsuPeople’s Republic of China
  3. 3.Eastern Hepatobiliary Surgery HospitalSecond Military Medical UniversityShanghaiPeople’s Republic of China