Cell Biology and Toxicology

, Volume 26, Issue 3, pp 265–277

Low-dose ethanol suppresses 17β-estradiol activity in GH4C1 pituitary tumor cells

  • Guoxin Wang
  • Dawei Chen
  • Haoshu Luo
  • Jiali Liu
  • Xiaowen Ji
  • Jingjing Fan
  • Sheng Cui
Article

DOI: 10.1007/s10565-009-9129-7

Cite this article as:
Wang, G., Chen, D., Luo, H. et al. Cell Biol Toxicol (2010) 26: 265. doi:10.1007/s10565-009-9129-7

Abstract

The interaction of chronic alcohol consumption with estrogen has been recognized in various tissues; however, the potential mechanism has not been clearly defined. In this study, we made the following observations: (a) 0.01% (v/v) ethanol (corresponding to 1.7 mM) significantly elevated estrogen receptor α (ERα) protein content, stimulated activator protein-1 (AP-1)-dependent ERα transcriptional activities, and ultimately enhanced GH4C1 cells growth in vitro and (b) the same concentration of ethanol suppressed the stimulatory effects of 17β-estradiol (E2; 10 nM) on both cell growth and cellular PRL accumulate through attenuation of ERα actions at both the estrogen response element and the AP-1 site. These observations raise the question as to what extent ethanol influences signal transduction pathways controlled by E2 in experimental medicine and biology.

Keywords

AP-1ERαEthanolGH4C1 cellsPituitary17β-estradiol

Abbreviations

AP-1

activator protein-1

BrdU

bromodeoxyuridine

EGF

epidermal growth factor

ER

estrogen receptor

ERE

estrogen response element

EtOH

ethanol

E2

17β-estradiol

GAPDH

glyceraldehyde-3-phosphate dehydrogenase

GFP

green fluorescent protein

GH4

GH4C1

HPRT

hypoxanthine phosphoribosyltransferase

IGF-1

insulin-like growth factor 1

MTT

methylthiazolyldiphenyl-tetrazolium bromide

PCR

polymerase chain reaction

PMSF

phenylmethanesulfonyl fluoride

PRL

prolactin

RNAi

RNA interference

shRNA

short hairpin RNA

SOE

splicing by overlap extension

TRITC

tetramethyl rhodamine isothiocyanate

Copyright information

© Springer Science+Business Media B.V. 2009

Authors and Affiliations

  • Guoxin Wang
    • 1
  • Dawei Chen
    • 1
  • Haoshu Luo
    • 1
  • Jiali Liu
    • 1
  • Xiaowen Ji
    • 1
  • Jingjing Fan
    • 1
  • Sheng Cui
    • 1
  1. 1.State Key Laboratories for AgroBiotechnology, College of Biological SciencesChina Agricultural UniversityHaidian District BeijingPeople’s Republic of China