Cell and Tissue Banking

, Volume 14, Issue 3, pp 413–422

Effectiveness of autologous serum as an alternative to fetal bovine serum in adipose-derived stem cell engineering

  • Jaehoon Choi
  • Jee-Hyeok Chung
  • Geun-Yong Kwon
  • Ki-Wan Kim
  • Sukwha Kim
  • Hak Chang
Original Paper

DOI: 10.1007/s10561-012-9341-1

Cite this article as:
Choi, J., Chung, JH., Kwon, GY. et al. Cell Tissue Bank (2013) 14: 413. doi:10.1007/s10561-012-9341-1
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Abstract

In cell culture, medium supplemented with fetal bovine serum is commonly used, and it is widely known that fetal bovine serum supplies an adequate environment for culture and differentiation of stem cells. Nevertheless, the use of xenogeneic serum can cause several problems. We compared the effects of four different concentrations of autologous serum (1, 2, 5, and 10 %) on expansion and adipogenic differentiation of adipose-derived stem cells using 10 % fetal bovine serum as a control. The stem cells were grafted on nude mice and the in vivo differentiation capacity was evaluated. The isolation of adipose-derived stem cells was successful irrespective of the culture medium. The proliferation potential was statistically significant at passage 2, as follows: 10 % autologous serum >10 % fetal bovine serum = 5 % autologous serum >2 % autologous serum = 1 % autologous serum. The differentiation capacity appeared statistically significant at passage 4, as follows: 10 % fetal bovine serum >10 % autologous serum = 5 % autologous serum >2 % autologous serum = 1 % autologous serum. Ten percent autologous serum and 10 % fetal bovine serum had greater differentiation capacity than 1 and 2 % autologous serum in vivo, and no significant difference was observed between the groups at ≥5 % concentration at 14 weeks. In conclusion, 10 % autologous serum was at least as effective as 10 % fetal bovine serum with respect to the number of adipose-derived stem cells at the end of both isolation and expansion, whereas 1 and 2 % autologous serum was inferior.

Keywords

Fetal bovine serum alternativeAutologous serumAdipose-derived stem cellAdipogenic differentiation

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Jaehoon Choi
    • 1
  • Jee-Hyeok Chung
    • 3
  • Geun-Yong Kwon
    • 4
  • Ki-Wan Kim
    • 2
  • Sukwha Kim
    • 2
  • Hak Chang
    • 2
  1. 1.Department of Plastic and Reconstructive Surgery, Gyeongsang National University HospitalGyeongsang National University School of MedicineJinjuRepublic of Korea
  2. 2.Department of Plastic and Reconstructive Surgery, Seoul National University College of MedicineSeoul National University HospitalSeoulRepublic of Korea
  3. 3.Profile Plastic Aesthetic ClinicSeoulRepublic of Korea
  4. 4.Division of Epidemic Intelligence ServiceKorea Centers for Disease Control and PreventionOsongRepublic of Korea