Cell and Tissue Banking

, Volume 14, Issue 2, pp 303–315

Plasticity and banking potential of cultured adipose tissue derived mesenchymal stem cells

  • M. Dhanasekaran
  • S. Indumathi
  • R. Poojitha
  • A. Kanmani
  • J. S. Rajkumar
  • D. Sudarsanam
Original Paper

DOI: 10.1007/s10561-012-9311-7

Cite this article as:
Dhanasekaran, M., Indumathi, S., Poojitha, R. et al. Cell Tissue Bank (2013) 14: 303. doi:10.1007/s10561-012-9311-7

Abstract

The present day research on stem cells is yet not filled to the gunwales. The correlation of stem cell technology with tissue repair still has a long way to go. Since Embryonic stem cells are a kind of thorn inside when it comes to therapeutics, there emerged few potent contemporary sources of stem cells. Though bone marrow proves to be the pioneer among these, they lose themselves to adipose tissue in various aspects. The major shortcoming of bone marrow lies in lieu of its loss in potency with age. Adipose tissue puts up a tough competition among leading edge stem cell sources like cord blood and cord matrix. Adipose tissue wins over its counterparts in that it possesses astounding proliferation potency in vitro and holds a prominent stand in showcasing in vivo tissue repair efficacy. In spite of its precedence, the whole enchilada of adipose derived stem cells is still in its salad days. In our work we aim at excogitating the Mesenchymal stem cell population present in cultured adipose derived stem cells, in a wide perspective. Furthermore, the coalition of cell adhesion molecules with the proliferation potency of MSC and analysis of growth curve of ADSC was also paid accolade. The presence of robust MSC with immense differentiation and transdifferentiation potency was endorsed by lucrative differentiation of P3 cells into mesodermal and neuronal lineages. Additionally, mesenchymal stem cells exhibiting coherent expression of surface markers at P3 in all samples can be cryopreserved for therapeutic applications.

Keywords

Mesenchymal stem cells Cell surface marker Cell culture Flowcytometry Cryopreservation 

Supplementary material

10561_2012_9311_MOESM1_ESM.doc (103 kb)
Supplementary material 1 (DOC 103 kb)

Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • M. Dhanasekaran
    • 1
  • S. Indumathi
    • 1
  • R. Poojitha
    • 2
  • A. Kanmani
    • 2
  • J. S. Rajkumar
    • 2
  • D. Sudarsanam
    • 1
  1. 1.Loyola CollegeNungambakkam, ChennaiIndia
  2. 2.Lifeline Multispeciality HospitalPerungudi, ChennaiIndia

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