Cell and Tissue Banking

, Volume 13, Issue 2, pp 341–351

Isolation of three important types of stem cells from the same samples of banked umbilical cord blood

Authors

    • Laboratory of Stem cell Research and ApplicationUniversity of Science, Vietnam National University
  • Vu Bich Ngoc
    • Laboratory of Stem cell Research and ApplicationUniversity of Science, Vietnam National University
  • Dang Hoang Lam
    • Laboratory of Stem cell Research and ApplicationUniversity of Science, Vietnam National University
  • Nguyen Thanh Tam
    • Laboratory of Stem cell Research and ApplicationUniversity of Science, Vietnam National University
  • Pham Quoc Viet
    • Laboratory of Stem cell Research and ApplicationUniversity of Science, Vietnam National University
  • Phan Kim Ngoc
    • Laboratory of Stem cell Research and ApplicationUniversity of Science, Vietnam National University
Article

DOI: 10.1007/s10561-011-9262-4

Cite this article as:
Phuc, P.V., Ngoc, V.B., Lam, D.H. et al. Cell Tissue Bank (2012) 13: 341. doi:10.1007/s10561-011-9262-4

Abstract

It is known that umbilical cord blood (UCB) is a rich source of stem cells with practical and ethical advantages. Three important types of stem cells which can be harvested from umbilical cord blood and used in disease treatment are hematopoietic stem cells (HSCs), mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs). Since these stem cells have shown enormous potential in regenerative medicine, numerous umbilical cord blood banks have been established. In this study, we examined the ability of banked UCB collected to produce three types of stem cells from the same samples with characteristics of HSCs, MSCs and EPCs. We were able to obtain homogeneous plastic rapidly-adherent cells (with characteristics of MSCs), slowly-adherent (with characteristics of EPCs) and non-adherent cells (with characteristics of HSCs) from the mononuclear cell fractions of cryopreserved UCB. Using a protocol of 48 h supernatant transferring, we successfully isolated MSCs which expressed CD13, CD44 and CD90 while CD34, CD45 and CD133 negative, had typical fibroblast-like shape, and was able to differentiate into adipocytes; EPCs which were CD34, and CD90 positive, CD13, CD44, CD45 and CD133 negative, adherent with cobble-like shape; HSCs which formed colonies when cultured in MethoCult medium.

Keywords

Banked bloodCryopreserved umbilical cord bloodEndothelial progenitor cellsMesenchymal stem cellsHematopoietic stem cellsUmbilical cord blood

Copyright information

© Springer Science+Business Media B.V. 2011