Breast Cancer Research and Treatment

, Volume 137, Issue 2, pp 373–382

MicroRNA-30c targets cytoskeleton genes involved in breast cancer cell invasion

  • Jessica Bockhorn
  • Kathy Yee
  • Ya-Fang Chang
  • Aleix Prat
  • Dezheng Huo
  • Chika Nwachukwu
  • Rachel Dalton
  • Simo Huang
  • Kaitlin E. Swanson
  • Charles M. Perou
  • Olufunmilayo I. Olopade
  • Michael F. Clarke
  • Geoffrey L. Greene
  • Huiping Liu
Preclinical study

DOI: 10.1007/s10549-012-2346-4

Cite this article as:
Bockhorn, J., Yee, K., Chang, YF. et al. Breast Cancer Res Treat (2013) 137: 373. doi:10.1007/s10549-012-2346-4

Abstract

Metastasis remains a significant challenge in treating cancer. A better understanding of the molecular mechanisms underlying metastasis is needed to develop more effective treatments. Here, we show that human breast tumor biomarker miR-30c regulates invasion by targeting the cytoskeleton network genes encoding twinfilin 1 (TWF1) and vimentin (VIM). Both VIM and TWF1 have been shown to regulate epithelial-to-mesenchymal transition. Similar to TWF1, VIM also regulates F-actin formation, a key component of cellular transition to a more invasive mesenchymal phenotype. To further characterize the role of the TWF1 pathway in breast cancer, we found that IL-11 is an important target of TWF1 that regulates breast cancer cell invasion and STAT3 phosphorylation. The miR-30c-VIM/TWF1 signaling cascade is also associated with clinical outcome in breast cancer patients.

Keywords

miR-30cBreast tumor invasionTWF1VIMIL-11

Supplementary material

10549_2012_2346_MOESM1_ESM.pdf (374 kb)
Supplementary material 1 (PDF 374 kb)

Copyright information

© Springer Science+Business Media New York 2012

Authors and Affiliations

  • Jessica Bockhorn
    • 1
    • 2
  • Kathy Yee
    • 1
  • Ya-Fang Chang
    • 1
  • Aleix Prat
    • 3
    • 7
  • Dezheng Huo
    • 4
  • Chika Nwachukwu
    • 5
  • Rachel Dalton
    • 1
  • Simo Huang
    • 1
  • Kaitlin E. Swanson
    • 1
  • Charles M. Perou
    • 3
  • Olufunmilayo I. Olopade
    • 5
  • Michael F. Clarke
    • 6
  • Geoffrey L. Greene
    • 1
  • Huiping Liu
    • 1
    • 6
  1. 1.The Ben May Department for Cancer ResearchThe University of ChicagoChicagoUSA
  2. 2.Department of Biochemistry and Molecular BiologyThe University of ChicagoChicagoUSA
  3. 3.Lineberger Comprehensive Cancer CenterThe University of North Carolina at Chapel HillChapel HillUSA
  4. 4.Department of Health StudiesThe University of ChicagoChicagoUSA
  5. 5.Center for Clinical Cancer Genetics, Department of MedicineThe University of ChicagoChicagoUSA
  6. 6.The Institute for Stem Cell Biology and Regenerative MedicineStanford UniversityStanfordUSA
  7. 7.Translational Genomics GroupVall D′Hebron Institute of Oncology (VHIO)BarcelonaSpain