Breast Cancer Research and Treatment

, Volume 125, Issue 2, pp 421–430

Enhanced serine production by bone metastatic breast cancer cells stimulates osteoclastogenesis

  • Sirkku Pollari
  • Sanna-Maria Käkönen
  • Henrik Edgren
  • Maija Wolf
  • Pekka Kohonen
  • Henri Sara
  • Theresa Guise
  • Matthias Nees
  • Olli Kallioniemi
Preclinical study

DOI: 10.1007/s10549-010-0848-5

Cite this article as:
Pollari, S., Käkönen, SM., Edgren, H. et al. Breast Cancer Res Treat (2011) 125: 421. doi:10.1007/s10549-010-0848-5

Abstract

Since bone metastatic breast cancer is an incurable disease, causing significant morbidity and mortality, an understanding of the underlying molecular mechanisms would be highly valuable. Here, we describe in vitro and in vivo evidences for the importance of serine biosynthesis in the metastasis of breast cancer to bone. We first characterized the bone metastatic propensity of the MDA-MB-231(SA) cell line variant as compared to the parental MDA-MB-231 cells by radiographic and histological observations in the inoculated mice. Genome-wide gene expression profiling of this isogenic cell line pair revealed that all the three genes involved in the l-serine biosynthesis pathway, phosphoglycerate dehydrogenase (PHGDH), phosphoserine aminotransferase 1 (PSAT1), and phosphoserine phosphatase (PSPH) were upregulated in the highly metastatic variant. This pathway is the primary endogenous source for l-serine in mammalian tissues. Consistently, we observed that the proliferation of MDA-MB-231(SA) cells in serine-free conditions was dependent on PSAT1 expression. In addition, we observed that l-serine is essential for the formation of bone resorbing human osteoclasts and may thus contribute to the vicious cycle of osteolytic bone metastasis. High expression of PHGDH and PSAT1 in primary breast cancer was significantly associated with decreased relapse-free and overall survival of patients and malignant phenotypic features of breast cancer. In conclusion, high expression of serine biosynthesis genes in metastatic breast cancer cells and the stimulating effect of l-serine on osteoclastogenesis and cancer cell proliferation indicate a functionally critical role for serine biosynthesis in bone metastatic breast cancer and thereby an opportunity for targeted therapeutic interventions.

Keywords

Breast cancerBone metastasisOsteoclastl-serine

Supplementary material

10549_2010_848_MOESM1_ESM.pdf (199 kb)
Supplementary material 1 (PDF 199 kb)

Copyright information

© Springer Science+Business Media, LLC. 2010

Authors and Affiliations

  • Sirkku Pollari
    • 1
  • Sanna-Maria Käkönen
    • 2
    • 5
  • Henrik Edgren
    • 3
  • Maija Wolf
    • 3
  • Pekka Kohonen
    • 1
  • Henri Sara
    • 1
  • Theresa Guise
    • 4
  • Matthias Nees
    • 1
  • Olli Kallioniemi
    • 3
  1. 1.Medical BiotechnologyVTT Technical Research Center of Finland and Turku Center for BiotechnologyTurkuFinland
  2. 2.Institute of Biomedicine, Department of AnatomyUniversity of TurkuTurkuFinland
  3. 3.Institute for Molecular Medicine Finland (FIMM)University of HelsinkiHelsinkiFinland
  4. 4.Division of EndocrinologyIndiana UniversityIndianapolisUSA
  5. 5.Amgen AB FinlandEspooFinland