Radiofrequency thermal ablation of breast tumors combined with intralesional administration of IL-7 and IL-15 augments anti-tumor immune responses and inhibits tumor development and metastasis

  • Mehran Habibi
  • Maciej Kmieciak
  • Laura Graham
  • Johanna K. Morales
  • Harry D. Bear
  • Masoud H. Manjili
Preclinical Study

DOI: 10.1007/s10549-008-0024-3

Cite this article as:
Habibi, M., Kmieciak, M., Graham, L. et al. Breast Cancer Res Treat (2009) 114: 423. doi:10.1007/s10549-008-0024-3

Abstract

Tumor development or recurrence is always a matter of concern following radiofrequency thermal ablation (RFA) of tumors. To determine whether combining RFA with immunologically active cytokines might induce tumor-specific immune responses against mammary carcinoma and inhibit tumor development or metastasis, we evaluated intralesional injection of IL-7 and IL-15 in RFA-treated murine tumors. We used two different breast carcinoma models: neu-overexpressing mouse mammary carcinoma (MMC) in FVBN202 transgenic mouse and 4T1 tumors in Balb/c mouse. MMC tend to relapse even in the presence of neu-specific immune responses, and 4T1 is a weakly immunogenic, aggressive and highly metastatic transplantable tumor. In vivo growth of both of these tumors is also associated with increased numbers of CD11b+Gr1+ myeloid-derived suppressor cells (MDSC). We showed for the first time that unlike RFA alone, RFA combined with the administration of intralesional IL-7 and IL-15 (after RFA), induced immune responses to tumors, inhibited tumor development and lung metastasis, and reduced MDSC.

Keywords

Radiofrequency thermal ablation (RFA) Breast cancer Immunotherapy Myeloid-derived suppressor cells (MDSC) Interleukin-7 (IL-7) Interleukin 15 (IL-15) 

Copyright information

© Springer Science+Business Media, LLC. 2008

Authors and Affiliations

  • Mehran Habibi
    • 1
  • Maciej Kmieciak
    • 1
  • Laura Graham
    • 1
  • Johanna K. Morales
    • 1
  • Harry D. Bear
    • 1
  • Masoud H. Manjili
    • 1
  1. 1.Department of Microbiology and ImmunologyVCU School of Medicine, Massey Cancer CenterRichmondUSA

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