Breast Cancer Research and Treatment

, Volume 110, Issue 2, pp 317–326

The prognostic value of Stathmin-1, S100A2, and SYK proteins in ER-positive primary breast cancer patients treated with adjuvant tamoxifen monotherapy: an immunohistochemical study

  • Rastko Golouh
  • Tanja Cufer
  • Aleksander Sadikov
  • Petra Nussdorfer
  • Pernille Autzen Usher
  • Nils Brünner
  • Manfred Schmitt
  • Ralf Lesche
  • Sabine Maier
  • Mieke Timmermans
  • John A. Foekens
  • John W. M. Martens
Clinical Trial

DOI: 10.1007/s10549-007-9724-3

Cite this article as:
Golouh, R., Cufer, T., Sadikov, A. et al. Breast Cancer Res Treat (2008) 110: 317. doi:10.1007/s10549-007-9724-3

Abstract

Introduction

We recently found that DNA methylation of S100A2, spleen tyrosine kinase (SYK), and Stathmin-1 (STMN1) correlates with response to tamoxifen therapy in metastatic breast cancer. In this retrospective study, we investigated immunohistochemically whether these three markers are predictors of relapse in early breast cancer (EBC) patients treated with adjuvant tamoxifen alone.

Methods

Immunohistochemical staining was performed for S100A2, SYK and STMN1 on a tissue microarray containing ER-positive invasive breast carcinomas from a study cohort of 215 operable breast cancer patients, who underwent radical local therapy and who were treated with adjuvant tamoxifen monotherapy. Cox regression was used to correlate staining intensity of the three markers with main endpoints in our study; disease-free survival (DFS), and disease-specific survival (DSS).

Results

In univariate analysis, only STMN1 staining intensity strongly correlated with DFS (P = 0.014) and DSS (P = 0.002). In the groups of low and high STMN1 intensity, DFS was 84% and 63%, and DSS was 89% and 70%. STMN1 retained its prognostic value for DFS (P = 0.002) and DSS (<0.001) in the multivariate model together with lymph node status. We found also a trend to better DFS in patients with low STMN1 intensity in both lymph node-positive (P = 0.001) and -negative patients (P = 0.065). As the tumour cells did not express S100A2 (except in one case) the potential prognostic value of this marker was not evaluated.

Conclusions

Staining intensity of STMN1, but not SYK, predicted outcome in our collective of ER- positive tamoxifen treated EBC patients.

Keywords

ERImmunohistochemistryPrimary breast carcinomaS100A2Stathmin-1SYKTamoxifen

Copyright information

© Springer Science+Business Media, LLC 2007

Authors and Affiliations

  • Rastko Golouh
    • 1
  • Tanja Cufer
    • 1
  • Aleksander Sadikov
    • 2
  • Petra Nussdorfer
    • 1
  • Pernille Autzen Usher
    • 3
  • Nils Brünner
    • 3
  • Manfred Schmitt
    • 4
  • Ralf Lesche
    • 5
  • Sabine Maier
    • 5
  • Mieke Timmermans
    • 6
  • John A. Foekens
    • 6
  • John W. M. Martens
    • 6
  1. 1.Institute of OncologyLjubljanaSlovenia
  2. 2.Artificial Intelligence LaboratoryUniversity of LjubljanaLjubljanaSlovenia
  3. 3.Royal Veterinary and Agriculture UniversityFrederiksbergDenmark
  4. 4.Department of Obstetrics and Gynaecology, Klinikum rechts der IsarTechnical University of MunichMunichGermany
  5. 5.Epigenomics AGBerlinGermany
  6. 6.Department of Medical OncologyErasmus MCRotterdamThe Netherlands