Predicting response to primary chemotherapy: gene expression profiling of paraffin-embedded core biopsy tissue
- Lida MinaAffiliated withDepartment of Medicine, Indiana University
- , Sharon E. SouleAffiliated withDivision of Hematology and Oncology, Indiana University
- , Sunil BadveAffiliated withDepartment of Anatomic Pathology, Indiana University
- , Fredrick L. BaehnerAffiliated withGenomic Health Inc.
- , Joffre BakerAffiliated withGenomic Health Inc.
- , Maureen CroninAffiliated withGenomic Health Inc.
- , Drew WatsonAffiliated withGenomic Health Inc.
- , Mei-Lan LiuAffiliated withGenomic Health Inc.
- , George W. SledgeJrAffiliated withDivision of Hematology and Oncology, Indiana University
- and 2 more
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Primary chemotherapy provides an ideal opportunity to correlate gene expression with response to treatment. We used paraffin-embedded core biopsies from a completed phase II trial to identify genes that correlate with response to primary chemotherapy.
Patients and Methods
Patients with newly diagnosed stage II or III breast cancer were treated with sequential doxorubicin 75 mg/M2 q2 wks × 3 and docetaxel 40 mg/M2 weekly × 6; treatment order was randomly assigned. Pretreatment core biopsy samples were interrogated for genes that might correlate with pathologic complete response (pCR). In addition to the individual genes, the correlation of the Oncotype DX Recurrence Score with pCR was examined.
Of 70 patients enrolled in the parent trial, core biopsies samples with sufficient RNA for gene analyses were available from 45 patients; 9 (20%) had inflammatory breast cancer (IBC). Six (14%) patients achieved a pCR. Twenty-two of the 274 candidate genes assessed correlated with pCR (p < 0.05). Genes correlating with pCR could be grouped into three large clusters: angiogenesis-related genes, proliferation related genes, and invasion-related genes. Expression of estrogen receptor (ER)-related genes and Recurrence Score did not correlate with pCR. In an exploratory analysis we compared gene expression in IBC to non-inflammatory breast cancer; twenty-four (9%) of the genes were differentially expressed (p < 0.05), 5 were upregulated and 19 were downregulated in IBC.
Gene expression analysis on core biopsy samples is feasible and identifies candidate genes that correlate with pCR to primary chemotherapy. Gene expression in IBC differs significantly from noninflammatory breast cancer.
KeywordsAngiogenesis Proliferation Invasion Inflammatory breast cancer
- Predicting response to primary chemotherapy: gene expression profiling of paraffin-embedded core biopsy tissue
Breast Cancer Research and Treatment
Volume 103, Issue 2 , pp 197-208
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- Kluwer Academic Publishers-Plenum Publishers
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- Inflammatory breast cancer
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- Author Affiliations
- 1. Department of Medicine, Indiana University, 535 Barnhill Drive, RT-473, Indianapolis, IN, 46202, USA
- 2. Division of Hematology and Oncology, Indiana University, 535 Barnhill Drive, RT-473, Indianapolis, IN, 46202, USA
- 3. Department of Anatomic Pathology, Indiana University, 535 Barnhill Drive, RT-473, Indianapolis, IN, 46202, USA
- 4. Genomic Health Inc., Redwood City, CA, USA