Biomedical Microdevices

, 18:63

A simple microfluidic device to study cell-scale endothelial mechanotransduction

  • Julie Lafaurie-Janvore
  • Elizabeth E. Antoine
  • Sidney J. Perkins
  • Avin Babataheri
  • Abdul I. Barakat
Article

DOI: 10.1007/s10544-016-0090-y

Cite this article as:
Lafaurie-Janvore, J., Antoine, E.E., Perkins, S.J. et al. Biomed Microdevices (2016) 18: 63. doi:10.1007/s10544-016-0090-y
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Abstract

Atherosclerosis is triggered by chronic inflammation of arterial endothelial cells (ECs). Because atherosclerosis develops preferentially in regions where blood flow is disturbed and where ECs have a cuboidal morphology, the interplay between EC shape and mechanotransduction events is of primary interest. In this work we present a simple microfluidic device to study relationships between cell shape and EC response to fluid shear stress. Adhesive micropatterns are used to non-invasively control EC elongation and orientation at both the monolayer and single cell levels. The micropatterned substrate is coupled to a microfluidic chamber that allows precise control of the flow field, high-resolution live-cell imaging during flow experiments, and in situ immunostaining. Using micro particle image velocimetry, we show that cells within the chamber alter the local flow field so that the shear stress on the cell surface is significantly higher than the wall shear stress in regions containing no cells. In response to flow, we observe the formation of lamellipodia in the downstream portion of the EC and cell retraction in the upstream portion. We quantify flow-induced calcium mobilization at the single cell level for cells cultured on unpatterned surfaces or on adhesive lines oriented either parallel or orthogonal to the flow. Finally, we demonstrate flow-induced intracellular calcium waves and show that the direction of propagation of these waves is determined by cell polarization rather than by the flow direction. The combined versatility and simplicity of this microfluidic device renders it very useful for studying relationships between EC shape and mechanosensitivity.

Keywords

Atherosclerosis Mechanobiology Shear stress Calcium signaling Micropatterns Microfluidic flow chamber 

Supplementary material

10544_2016_90_MOESM1_ESM.pdf (355 kb)
ESM 1(PDF 355 kb)
10544_2016_90_MOESM2_ESM.avi (21 mb)
ESM 2(AVI 21475 kb)
10544_2016_90_MOESM3_ESM.avi (18.6 mb)
ESM 3(AVI 19085 kb)
10544_2016_90_MOESM4_ESM.avi (283 kb)
ESM 4(AVI 283 kb)

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Julie Lafaurie-Janvore
    • 1
  • Elizabeth E. Antoine
    • 1
  • Sidney J. Perkins
    • 1
    • 2
  • Avin Babataheri
    • 1
  • Abdul I. Barakat
    • 1
  1. 1.Laboratoire d’Hydrodynamique de l’École polytechniqueCNRS-EP UMR 7646PalaiseauFrance
  2. 2.Columbia UniversityNew YorkUSA

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