Biomedical Microdevices

, Volume 13, Issue 3, pp 585–595

A suction-type microfluidic immunosensing chip for rapid detection of the dengue virus

  • Chen-Hsun Weng
  • Tze-Bin Huang
  • Chih-Chia Huang
  • Chen-Sheng Yeh
  • Huan-Yao Lei
  • Gwo-Bin Lee
Article

DOI: 10.1007/s10544-011-9529-3

Cite this article as:
Weng, C., Huang, T., Huang, C. et al. Biomed Microdevices (2011) 13: 585. doi:10.1007/s10544-011-9529-3

Abstract

The enzyme-linked immunosorbent assay (ELISA) is widely used in medical diagnostics. In order to reduce the diagnosis time and to lower the consumption of sample/reagents in an ELISA assay, a suction-type, automatic, pneumatically-driven microfluidic chip has been designed and fabricated in this study. The microfluidic chip integrates a multi-functional micro-transport/mixing unit, for transporting metering and mixing of samples and reagents in order to automatically perform the entire ELISA protocol. A new surface modification has been adopted which allows for a high processing capacity. The detection sensitivity for the dengue virus is found to be 101 PFU/ml, which is much better than a conventional ELISA assay (103 PFU/ml). The entire assay time is only 30 min, which is much faster than with 96-well microtiter plates (4 h). The consumed sample and reagent volume is only 12 μl, which is less than a conventional assay (100 μl). The development of this microfluidic chip may be promising for other immunosensing applications.

Keywords

MicrofluidicsSuctionImmunosensingELISADengue virusSurface modification

Nomenclature

BSA

Bovine serum albumin

CCD

Charge-coupled device

DI

Deionized

DV

Dengue virus

ELISA

Enzyme-linked immunosorbent assay

EMV

Electromagnetic valve

EV

Enterovirus

GA

Glutaraldehyde

HAU

Hemagglutination unit

HBV

Hepatitis B virus

HRP

Horse reddish peroxidase

IgG

Immunoglobulin G

IU

International unit

LBL

Layer-by-layer

OD

Optical density

PAA

Poly(acrylic acid)

PBS

Phosphate buffer saline

PDMS

Polydimethylsiloxane

PEI

Poly(ethyleneimine)

PEM

Polyelectrolyte multilayer

PFU

Plaque forming unit

Pluronic P123

Poly (ethylene oxide)-poly (propylene oxide)-poly (ethylene oxide) triblock copolymer

RT-PCR

Reverse-transcription polymerase chain reaction

TMB

Tetramethylbenzidin

UV

Ultraviolet

Copyright information

© Springer Science+Business Media, LLC 2011

Authors and Affiliations

  • Chen-Hsun Weng
    • 3
  • Tze-Bin Huang
    • 1
  • Chih-Chia Huang
    • 2
  • Chen-Sheng Yeh
    • 2
  • Huan-Yao Lei
    • 3
  • Gwo-Bin Lee
    • 4
  1. 1.Department of Engineering ScienceNational Cheng Kung UniversityTainanTaiwan
  2. 2.Department of ChemistryNational Cheng Kung UniversityTainanTaiwan
  3. 3.Department of Microbiology and ImmunologyNational Cheng Kung UniversityTainanTaiwan
  4. 4.Department of Power Mechanical EngineeringNational Tsing Hua UniversityHsinchuTaiwan