Biomedical Microdevices

, Volume 12, Issue 6, pp 987–1000

Microfluidic device to study arterial shear-mediated platelet-surface interactions in whole blood: reduced sample volumes and well-characterised protein surfaces

  • Nigel J. Kent
  • Lourdes Basabe-Desmonts
  • Gerardene Meade
  • Brian D. MacCraith
  • Brian G. Corcoran
  • Dermot Kenny
  • Antonio J. Ricco
Article

DOI: 10.1007/s10544-010-9453-y

Cite this article as:
Kent, N.J., Basabe-Desmonts, L., Meade, G. et al. Biomed Microdevices (2010) 12: 987. doi:10.1007/s10544-010-9453-y

Abstract

We report a novel device to analyze cell-surface interactions under controlled fluid-shear conditions on well-characterised protein surfaces. Its performance is demonstrated by studying platelets interacting with immobilised von Willebrand Factor at arterial vascular shear rates using just 200 μL of whole human blood per assay. The device’s parallel-plate flow chamber, with 0.1 mm2 cross sectional area and height-to-width ratio of 1:40, provides uniform, well-defined shear rates along the chip surface with negligible vertical wall effects on the fluid flow profile while minimizing sample volumetric flow. A coating process was demonstrated by ellipsometry, atomic force microscopy, and fluorescent immunostaining to provide reproducible, homogeneous, uniform protein layers over the 0.7 cm2 cell-surface interaction area. Customized image processing quantifies dynamic cellular surface coverage vs. time throughout the whole-blood-flow assay for a given drug treatment or disease state. This device can track the dose response of anti-platelet drugs, is suitable for point-of-care diagnostics, and is designed for adaptation to mass manufacture.

Keywords

Shear activationMicrofluidicsPlatelet-surface interactionsProtein characterisationImage analysis

Supplementary material

10544_2010_9453_Fig8_ESM.jpg (259 kb)
Fig. S1

3D height AFM images of 2 × 2 μm2 areas of VWF protein layers on SiO2-on-Si wafers for 2-hr adsorption from aqueous VWF solutions: 5, 50, 100, and 500 μg/mL. These correspond to the four 2D images shown in Figure 6(c). (JPEG 259 kb)

10544_2010_9453_MOESM1_ESM.tif (12.5 mb)
High Resolution Image (TIFF 12797 kb)

Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Nigel J. Kent
    • 1
    • 4
  • Lourdes Basabe-Desmonts
    • 1
  • Gerardene Meade
    • 2
  • Brian D. MacCraith
    • 1
  • Brian G. Corcoran
    • 3
  • Dermot Kenny
    • 2
  • Antonio J. Ricco
    • 1
  1. 1.Biomedical Diagnostics InstituteDublin City UniversityGlasnevinIreland
  2. 2.Biomedical Diagnostics Institute, Dept of Molecular and Cellular TherapeuticsRoyal College of Surgeons in IrelandDublin 2Ireland
  3. 3.School of Mechanical and Manufacturing EngineeringDublin City UniversityGlasnevinIreland
  4. 4.The Biomedical Devices and Assistive Technology Research Group, College of Engineering and Built Environment, Dublin Institute of TechnologyDublin 1Ireland