Biomedical Microdevices

, Volume 12, Issue 2, pp 353–359

An integrated disposable device for DNA extraction and helicase dependent amplification

  • Madhumita Mahalanabis
  • Jaephil Do
  • Hussam ALMuayad
  • Jane Y. Zhang
  • Catherine M. Klapperich
Article

DOI: 10.1007/s10544-009-9391-8

Cite this article as:
Mahalanabis, M., Do, J., ALMuayad, H. et al. Biomed Microdevices (2010) 12: 353. doi:10.1007/s10544-009-9391-8

Abstract

Here we report the demonstration of an integrated microfluidic chip that performs helicase dependent amplification (HDA) on samples containing live bacteria. Combined chip-based sample preparation and isothermal amplification are attractive for world health applications, since the need for instrumentation to control flow rate and temperature changes are reduced or eliminated. Bacteria lysis, nucleic acid extraction, and DNA amplification with a fluorescent reporter are incorporated into a disposable polymer cartridge format. Smart passive fluidic control using a flap valve and a hydrophobic vent (with a nanoporous PTFE membrane) with a simple on-chip mixer eliminates multiple user operations. The device is able to detect as few as ten colony forming units (CFU) of E. coli in growth medium.

Keywords

DNABacteriaHelicase dependent amplificationIsothermal amplificationMicrofluidic chipPlasticSolid phase extraction

Copyright information

© Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Madhumita Mahalanabis
    • 1
  • Jaephil Do
    • 1
  • Hussam ALMuayad
    • 1
  • Jane Y. Zhang
    • 1
  • Catherine M. Klapperich
    • 1
    • 2
  1. 1.Department of Biomedical EngineeringBoston UniversityBostonUSA
  2. 2.Department of Mechanical EngineeringBoston UniversityBostonUSA